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Complete Human and Rat Ex Vivo Spermatogenesis from Fresh or Frozen Testicular Tissue
- Source :
- Biology of Reproduction, Biology of Reproduction, Society for the Study of Reproduction, 2016, 95, ⟨10.1095/biolreprod.116.142802⟩, Biology of Reproduction, 2016, 95 (4), pp.89-89. ⟨10.1095/biolreprod.116.142802⟩, Biology of Reproduction, Society for the Study of Reproduction, 2016, 95 (4), pp.89-89. ⟨10.1095/biolreprod.116.142802⟩
- Publication Year :
- 2016
- Publisher :
- Oxford University Press (OUP), 2016.
-
Abstract
- International audience; Until now, complete ex vivo spermatogenesis has been reported only in the mouse. In this species, the duration of spermatogenesis is 35 days, whereas it is 54 days in the rat and 74 days in humans. We performed long-term (until 60 days) cultures of fresh or frozen rat or human seminiferous tubule segments in a bioreactor made of a hollow cylinder of chitosan hydrogel. Testicular tissues were obtained from 8- or 20-day-old male rats or from adult human subjects who had undergone hormone treatments leading to a nearly complete regression of their spermatogenesis before bilateral orchiectomy for gender reassignment. The progression of spermatogenesis was assessed by cytological analyses of the cultures; it was related to a dramatic increase in the levels of the mRNAs specifically expressed by round spermatids, Transition protein 1, Transition protein 2, and Protamine 3 in rat cultures. From 2% to 3.8% of cells were found to be haploid cells by fluorescence in situ hybridization analysis of human cultures. In this bioreactor, long-term cultures of seminiferous tubule segments from prepubertal rats or from adult men allowed completion of the spermatogenic process leading to morphologically mature spermatozoa. Further studies will need to address the way of optimizing the yield of every step of spermatogenesis by adjusting the composition of the culture medium, the geometry, and the material properties of the chitosan hydrogel bioreactors. Another essential requirement is to assess the quality of the gametes produced ex vivo by showing their ability to produce normal offspring (rat) or their biochemical normality (human).
- Subjects :
- Male
hollow cylinder
0301 basic medicine
Cryopreservation
Rats, Sprague-Dawley
bioreactor
Bioreactors
0302 clinical medicine
Testis
In Situ Hybridization, Fluorescence
ComputingMilieux_MISCELLANEOUS
[SDV.BDD.GAM]Life Sciences [q-bio]/Development Biology/Gametogenesis
030219 obstetrics & reproductive medicine
medicine.diagnostic_test
Hydrogels
[CHIM.MATE]Chemical Sciences/Material chemistry
General Medicine
Seminiferous Tubules
Spermatids
Seminiferous tubule
medicine.anatomical_structure
Adult
medicine.medical_specialty
fertility preservation
Offspring
In Vitro Techniques
Biology
human spermatogenesis
03 medical and health sciences
Species Specificity
Internal medicine
medicine
Animals
Humans
RNA, Messenger
Spermatogenesis
[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials
Cell Biology
Protamine
Culture Media
Rats
[CHIM.POLY]Chemical Sciences/Polymers
030104 developmental biology
Endocrinology
Reproductive Medicine
biology.protein
chitosan
Ex vivo
Fluorescence in situ hybridization
Hormone
Subjects
Details
- ISSN :
- 15297268 and 00063363
- Volume :
- 95
- Database :
- OpenAIRE
- Journal :
- Biology of Reproduction
- Accession number :
- edsair.doi.dedup.....f42ae0d486bde38aadeaa0df867e0175