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Fast Cleavage Kinetics of a Natural Hammerhead Ribozyme
- Source :
- Journal of the American Chemical Society. 126:10848-10849
- Publication Year :
- 2004
- Publisher :
- American Chemical Society (ACS), 2004.
-
Abstract
- The hammerhead ribozyme is a small RNA motif that catalyzes the cleavage and ligation of RNA. The well-studied minimal hammerhead motif is inactive under physiological conditions and requires high Mg(2+) concentrations for efficient cleavage. In contrast, natural hammerheads are active under physiological conditions and contain motifs outside the catalytic core that lower the requirement for Mg(2+). Single-turnover kinetics were used here to characterize the Mg(2+) and pH dependence for cleavage of a trans-cleaving construct of the Schistosoma mansoni natural hammerhead ribozyme. Compared to the minimal hammerhead motif, the natural Schistosoma ribozyme requires 100-fold less Mg(2+) to achieve a cleavage rate of 1 min(-1). The improved catalysis results from tertiary interactions between loops in stems I and II and likely arises from increasing the population of the active conformation. Under optimum pH and Mg(2+) conditions this ribozyme cleaves at over 870 min(-1) at 25 degrees C, further demonstrating the impressive catalytic power of this ribozyme.
- Subjects :
- Hammerhead ribozyme
Stereochemistry
Population
Cleavage (embryo)
Biochemistry
Catalysis
Colloid and Surface Chemistry
Animals
Magnesium
RNA, Catalytic
education
education.field_of_study
biology
Chemistry
Ribozyme
General Chemistry
Hydrogen-Ion Concentration
biology.organism_classification
Kinetics
enzymes and coenzymes (carbohydrates)
GlmS glucosamine-6-phosphate activated ribozyme
biology.protein
Schistosoma
Mammalian CPEB3 ribozyme
Hairpin ribozyme
VS ribozyme
Subjects
Details
- ISSN :
- 15205126 and 00027863
- Volume :
- 126
- Database :
- OpenAIRE
- Journal :
- Journal of the American Chemical Society
- Accession number :
- edsair.doi.dedup.....f425fd5ceb25cc8a5dc365881a7a0209