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A new regulatory principle for in vivo biochemistry: pleiotropic low affinity regulation by the adenine nucleotides--illustrated for the glycolytic enzymes of Saccharomyces cerevisiae
- Source :
- FEBS Letters, 587(17), 2860-2867. Wiley, FEBS Letters, 587(17), 2860-2867. Wiley-Blackwell, Mensonides, F I C, Bakker, B M, Cremazy, F, Messiha, H L, Mendes, P, Boogerd, F C & Westerhoff, H V 2013, ' A new regulatory principle for in vivo biochemistry : pleiotropic low affinity regulation by the adenine nucleotides--illustrated for the glycolytic enzymes of Saccharomyces cerevisiae ', FEBS Letters, vol. 587, no. 17, pp. 2860-2867 . https://doi.org/10.1016/j.febslet.2013.07.013, FEBS Letters, 587(17), 2860-2867. Elsevier
- Publication Year :
- 2013
-
Abstract
- Enzymology tends to focus on highly specific effects of substrates, allosteric modifiers, and products occurring at low concentrations, because these are most informative about the enzyme's catalytic mechanism. We hypothesized that at relatively high in vivo concentrations, important molecular monitors of the state of living cells, such as ATP, affect multiple enzymes of the former and that these interactions have gone unnoticed in enzymology.We test this hypothesis in terms of the effect that ATP, ADP, and AMP might have on the major free-energy delivering pathway of the yeast Saccharomyces cerevisiae. Assaying cell-free extracts, we collected a comprehensive set of quantitative kinetic data concerning the enzymes of the glycolytic and the ethanol fermentation pathways. We determined systematically the extent to which the enzyme activities depend on the concentrations of the adenine nucleotides. We found that the effects of the adenine nucleotides on enzymes catalysing reactions in which they are not directly involved as substrate or product, are substantial. This includes effects on the Michaelis-Menten constants, adding new perspective on these, 100 years after their introduction. (C) 2013 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
- Subjects :
- Biochemistry
GLUCOSE
chemistry.chemical_compound
Structural Biology
Adenine nucleotide
Models
Glycolysis
NETWORK
Non-U.S. Gov't
chemistry.chemical_classification
0303 health sciences
biology
Adenine Nucleotides
Research Support, Non-U.S. Gov't
Systems Biology
YEAST HEXOKINASE
CHEMOSTAT
RESPIRATION
Thermodynamics
Phosphofructokinase
Subcellular Fractions
Yeast glycolysis model
Saccharomyces cerevisiae Proteins
Allosteric regulation
Saccharomyces cerevisiae
Biophysics
Research Support
Models, Biological
03 medical and health sciences
Allosteric Regulation
Energetics
Genetics
Journal Article
Enzyme kinetics
Molecular Biology
KINETICS
030304 developmental biology
030306 microbiology
Cell Biology
biology.organism_classification
Biological
ATP
Kinetics
Enzyme
chemistry
Fermentation
Glyceraldehyde 3-phosphate
Subjects
Details
- Language :
- English
- ISSN :
- 00145793
- Volume :
- 587
- Issue :
- 17
- Database :
- OpenAIRE
- Journal :
- FEBS Letters
- Accession number :
- edsair.doi.dedup.....f41f612590ff386721b67022db0eff5d