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Analysis of isorhamnetin-3-O-neohesperidoside in rat plasma by liquid chromatography/electrospray ionization tandem mass spectrometry and its application to pharmacokinetic studies
- Source :
- Chinese journal of natural medicines. 11(5)
- Publication Year :
- 2012
-
Abstract
- To establish an LC-MS/MS method for determination of isorhamnetin-3-O-neohesperidoside and investigate its application on pharmacokinetic study in rats.Eight rats were given 5 mg·kg(-1) isorhamnetin-3-O-neohesperidoside after intravenous administration. A highly sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of isorhamnetin-3-O-neohesperidosidein rat plasma using rutin as internal standard. The analytes and rutin (internal standard) were extracted with methanol followed by a rapid isocratic elution with 10 mmol·L(-1) ammonium acetate buffer/methanol (20 : 80, V/V) on a C18 column (150 mm × 2.1 mm, I.D., 5 μm) and subsequent analysis by mass spectrometry in the multi-eaction-monitoring mode.The assays were linear over the concentration range of 0.01-10 μg·mL(-1) for isorhamnetin-3-O-neohesperidosidein rat plasma. The lower limit of quantifications for isorhamnetin-3-O-neohesperidoside was 0.01 μg·mL(-1).The validated method is successfully applied to determine the plasma concentrations of isorhamnetin-3-O-neohesperidosidein in rats.
- Subjects :
- Spectrometry, Mass, Electrospray Ionization
Chromatography
Flavonols
Chemistry
Electrospray ionization
General Medicine
Plasma
Typhaceae
Rats
Liquid chromatography electrospray ionization tandem mass spectrometry
Complementary and alternative medicine
Pharmacokinetics
Isorhamnetin-3-O-neohesperidoside
Tandem Mass Spectrometry
Drug Discovery
Lc ms ms
Animals
Pollen
Chromatography, High Pressure Liquid
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 18755364
- Volume :
- 11
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Chinese journal of natural medicines
- Accession number :
- edsair.doi.dedup.....f401c6c41049b5abe0b3f89d6fb32a00