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Isolation, purification, structural elucidation and antimicrobial activities of kocumarin, a novel antibiotic isolated from actinobacterium Kocuria marina CMG S2 associated with the brown seaweed Pelvetia canaliculata
- Source :
- Microbiological research. 206
- Publication Year :
- 2017
-
Abstract
- Aims Screening of seaweed-associated bacteria capable of producing antimicrobials. Methods and results Fifteen microbial strains, associated to the brown seaweed Pelvetia canaliculata (Linnaeus) attached to the rocks of Sonmiani Beach (Karachi, Pakistan), were screened. Crude extract filtrates of CMG S2 strain grew on Zobell marine agar (ZMA) had the most remarkable antimicrobial activity. Based on its phenotypic aspects (e.g. Gram-positive, microccoid form), biochemical characteristics (e.g. halotolerance) and genetic analyses, CMG S2 is identified as a putatively new Kocuria marina type strain belonging to the actinobacteria’s class and micrococcaceae family. Thereby, the nucleotide sequence analysis of its full-length 16S ribosomal ribonucleic acid (rRNA) gene (GenBank accession number EU073966.1 ) displayed highest identity (i.e. 99%) and score (2630) with K. marina KMM 3905. Phylogenic trees analysis using the neighbor-joining method showed closest evolutionary distance of CMG S2 with KMM 3905 strain and K. carniphila (DC2201) specie. Interestingly, a unique ultraviolet (UV)-bioactive compound was purified from CMG S2 crude extracts by flash silica gel column and thin-layer chromatography (TLC) techniques. Its chemical structure was unraveled as 4-[(Z)-2 phenyl ethenyl] benzoic acid (PEBA, later named kocumarin) by nuclear magnetic resonance (NMR) spectroscopy techniques. Importantly, kocumarin demonstrated prominent and rapid growth inhibition against all tested fungi and pathogenic bacteria including methicillin-resistant Staphylococcus aureus (MRSA), with a minimal fungal inhibitory concentration (MFC) of 15–25 μg/mL and a minimal (bacterial) inhibitory concentration (MIC) of 10–15 μg/mL. Significance and impact of the study Kocumarin represents a new promising natural antibiotic for in vivo and environmental applications.
- Subjects :
- 0301 basic medicine
DNA, Bacterial
Methicillin-Resistant Staphylococcus aureus
food.ingredient
Magnetic Resonance Spectroscopy
030106 microbiology
Secondary Metabolism
Microbial Sensitivity Tests
medicine.disease_cause
Phaeophyta
Microbiology
Pelvetia
Actinobacteria
03 medical and health sciences
food
Species Specificity
RNA, Ribosomal, 16S
medicine
Agar
Pakistan
Phylogeny
biology
Bacteria
Fungi
Pathogenic bacteria
Sequence Analysis, DNA
Ribosomal RNA
biology.organism_classification
Antimicrobial
Seaweed
Anti-Bacterial Agents
Bacterial Typing Techniques
Kinetics
030104 developmental biology
Phenotype
Staphylococcus aureus
Fermentation
Chromatography, Thin Layer
Micrococcaceae
Subjects
Details
- ISSN :
- 16180623
- Volume :
- 206
- Database :
- OpenAIRE
- Journal :
- Microbiological research
- Accession number :
- edsair.doi.dedup.....f3f92078646b914aed96492f5b5c77de