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A member of cation diffusion facilitator family, MTP11, is required for manganese tolerance and high fertility in rice
- Source :
- Planta. 248(1)
- Publication Year :
- 2018
-
Abstract
- Rice MTP11 is the trans-Golgi-localized transporter that is involved in Mn tolerance with MTP8.1, and it is required for normal fertility. Rice (Oryza sativa L.) is one of the most manganese (Mn)-tolerant species, and it is able to accumulate high levels of this metal in the leaves without showing toxic symptoms. The metal tolerance protein 8.1 (MTP8.1), a member of the Mn-cation diffusion facilitator (CDF) family, has been shown to play a central role in high Mn tolerance by sequestering Mn into vacuoles. Recently, rice MTP11 was identified as an Mn transporter that is localized to Golgi-associated compartments, but its exact role in Mn tolerance in planta has not yet been understood. Here, we investigated the role of MTP11 in rice Mn tolerance using knockout lines. Old leaves presented higher levels of constitutively expressed MTP11 than other tissues and MTP11 expression was also found in reproductive organs. Fused MTP11:green fluorescent protein was co-localized to trans-Golgi markers and differentiated from other Golgi-associated markers. Knockout of MTP11 in wild-type rice did not affect tolerance and accumulation of Mn and other heavy metals, but knockout in the mtp8.1 mutant showed exacerbated Mn sensitivity at the vegetative growth stage. Knockout of MTP11 alone resulted in decreased grain yield and fertility at the reproductive stage. Thus, MTP11 is a trans-Golgi localized transporter for Mn, which plays a role in Mn tolerance through intracellular Mn compartmentalization. It is also required for maintaining high fertility in rice.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Mutant
Golgi Apparatus
Plant Science
Vacuole
Real-Time Polymerase Chain Reaction
01 natural sciences
Plant Roots
Green fluorescent protein
03 medical and health sciences
Genetics
Cation Transport Proteins
Plant Proteins
Manganese
Oryza sativa
Chemistry
Protoplasts
food and beverages
Transporter
Oryza
Sequence Analysis, DNA
Compartmentalization (fire protection)
Cell biology
030104 developmental biology
Fertility
Gene Knockdown Techniques
Intracellular
010606 plant biology & botany
Cation diffusion facilitator
Subjects
Details
- ISSN :
- 14322048
- Volume :
- 248
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Planta
- Accession number :
- edsair.doi.dedup.....f3f8b41dca12d4a9f138881730cdd10c