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Evaluation of the Higher Order Structure of Biotherapeutics Embedded in Hydrogels for Bioprinting and Drug Release
- Source :
- Analytical Chemistry
- Publication Year :
- 2021
-
Abstract
- Biocompatible hydrogels for tissue regeneration/replacement and drug release with specific architectures can be obtained by three-dimensional bioprinting techniques. The preservation of the higher order structure of the proteins embedded in the hydrogels as drugs or modulators is critical for their biological activity. Solution nuclear magnetic resonance (NMR) experiments are currently used to investigate the higher order structure of biotherapeutics in comparability, similarity, and stability studies. However, the size of pores in the gel, protein–matrix interactions, and the size of the embedded proteins often prevent the use of this methodology. The recent advancements of solid-state NMR allow for the comparison of the higher order structure of the matrix-embedded and free isotopically enriched proteins, allowing for the evaluation of the functionality of the material in several steps of hydrogel development. Moreover, the structural information at atomic detail on the matrix–protein interactions paves the way for a structure-based design of these biomaterials.
- Subjects :
- Tissue Engineering
Tissue Scaffolds
Chemistry
Bioprinting
Hydrogels
Nanotechnology
02 engineering and technology
010402 general chemistry
021001 nanoscience & nanotechnology
Biocompatible material
01 natural sciences
Article
0104 chemical sciences
Analytical Chemistry
Drug Liberation
Printing, Three-Dimensional
Tissue scaffolds
Tissue engineering
Three-Dimensional
Self-healing hydrogels
Drug release
Printing
0210 nano-technology
Higher Order Structure
Subjects
Details
- ISSN :
- 00032700
- Database :
- OpenAIRE
- Journal :
- Analytical Chemistry
- Accession number :
- edsair.doi.dedup.....f3aece958d4494dfd039741bb7461cbe
- Full Text :
- https://doi.org/10.1021/acs.analchem.1c01850