Ultrasonically synthesis of Mn- and Cu- @ ZnS-NPs-AC based ultrasound assisted extraction procedure and validation of a spectrophotometric method for a rapid preconcentration of Allura Red AC (E129) in food and water samples

This study is devoted on Allura Red as food colorant preconcentration and determination in beverage, fruit juice and drink water samples is based on usage of Mn- and Cu- @ ZnS-NPs-AC as new sorbent for ultrasound-assisted-dispersive solid-phase microextraction (UA-DSPME) combined with ultraviolet–visible spectrophotometric based method (UV–Vis). Contribution of volume of eluent, pH, sorbent mass and sonication time on response following conduction of 28 experiments were optimized and investigated while their significantly justified according to p-value. Values of “Prob > F” less than 0.0500 is proportional with their significant influence on recovery of analyte. Under the optimum conditions 0.14 mL of THF; pH of 2.5; 8 mg of sorbent and 3 min sonication time guide and help achievement of limit of quantification (LOQ) and limit of detection (LOD) of 6.08 and 20.26 ng mL−1, respectively. The accuracy of method was validated according to calculation of recovery following spiking 400 and 600 ng mL−1 to blank solution and recovery as more reliable indication of accuracy 93.41 and 102.17% recoveries with RSD