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Molecular characterization of a cell wall-associated ß(1-3)endoglucanase ofAspergillus fumigatus
- Source :
- Medical Mycology, Medical Mycology, Oxford University Press, 2002, 40 (5), pp.455-64. ⟨10.1080/mmy.40.5.455.464⟩, Medical Mycology, 2002, 40 (5), pp.455-64. ⟨10.1080/mmy.40.5.455.464⟩
- Publication Year :
- 2002
- Publisher :
- Oxford University Press (OUP), 2002.
-
Abstract
- International audience; A 74 kDa beta(1-3)endoglucanase of Aspergillus fumigatus was recently isolated from a cell wall autolysate and biochemically characterized. In this study, we report the cloning and the disruption of the ENGL1 gene encoding this beta(1-3)endoglucanase. ENGL1 contains an open reading frame of 2181 bp encoding a polypeptide of 727 amino acids. Sequence analysis showed that ENGL1 is the first characterized member of a new family of beta(1-3)glucanases. Disruption of ENGL1, however, did not lead to a phenotype distinct from the parental strain, indicating that this cell wall-associated beta(1-3)endoglucanase does not play an essential role in constitutive cell growth.
- Subjects :
- Sequence analysis
[SDV]Life Sciences [q-bio]
Molecular Sequence Data
MESH: Amino Acid Sequence
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
MESH: Phenotype
Aspergillus fumigatus
Cell wall
03 medical and health sciences
MESH: Cell Wall
Cell Wall
Amino Acid Sequence
Gene
[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
MESH: Molecular Sequence Data
biology
030306 microbiology
Cell growth
Glucan Endo-1,3-beta-D-Glucosidase
Nucleic acid sequence
General Medicine
biology.organism_classification
MESH: Glucan Endo-1,3-beta-D-Glucosidase
Amino acid
Open reading frame
Phenotype
Infectious Diseases
Biochemistry
chemistry
MESH: Aspergillus fumigatus
Subjects
Details
- ISSN :
- 14602709, 13693786, and 1365280X
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- Medical Mycology
- Accession number :
- edsair.doi.dedup.....f39554274ce7bbfec059704a10d2c2d2