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Caracterización biológica y molecular de cepas de Panstrongylus sp. nativas del Perú
- Source :
- Anales de la Facultad de Medicina, Vol 81, Iss 2 (2020)
- Publication Year :
- 2020
- Publisher :
- Universidad Nacional Mayor de San Marcos, 2020.
-
Abstract
- espanolObjetivo. Determinar las caracteristicas biologicas y moleculares de las cepas de Panstrongylussp. nativas del norte del Peru. Metodos. Estudio observacional, descriptivo. Se utilizo material biologico procedente de 5 diferentes regiones del norte del Peru, y por muestreo intencional se selecciono una muestra de 50 especimenes. Para la caracterizacion de los especimenes a nivel molecular, se uso el marcador ITS-2 del ADN ribosomal, mediante la tecnica del PCR. Resultados. El 100% de las muestras fueron clasificadas como Panstrongylus herreri. A nivel molecular se observaron 3 patrones diferentes de la banda ITS-2: 960pb, 800pb y 750pb. Conclusion. 100% de las muestras fueron Panstrongylus herreri, capturados en ambientes intradomiciliarios de regiones del norte del Peru, con un patron de ITS-2 EnglishObjective. To determine the biological and molecular characteristics of the Panstrongylus sp. native to northern Peru. Methods. Observational, descriptive study. Biological material from 5 different regions of northern Peru was used, and a sample of 50 specimens was selected by intentional sampling. For the characterization of the specimens at the molecular level, the ITS-2 marker of ribosomal DNA was used, using the PCR technique. Results. 100% of the samples were classified as Panstrongylus herreri. At the molecular level, 3 different patterns of the ITS-2 band were observed: 960bp, 800bp and 750bp. Conclusion. 100% of the samples were Panstrongylus herreri, captured in intra-household environments in northern regions of Peru, with an ITS-2 pattern.
Details
- Language :
- Spanish; Castilian
- ISSN :
- 16099419 and 10255583
- Volume :
- 81
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Anales de la Facultad de Medicina
- Accession number :
- edsair.doi.dedup.....f38801d5ffdc9efcb7bec52f664602e0