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Anionic Quantum Dots reveal actin-microridges in zebrafish epidermis
- Source :
- CONICET Digital (CONICET), Consejo Nacional de Investigaciones Científicas y Técnicas, instacron:CONICET
- Publication Year :
- 2020
-
Abstract
- Enhancement of hydrophilicity and functionalization of CdTe QDs (Quantum Dots) via surface modifications have made them suitable to be used as specific probes for cell imaging. Applications for targeting cell surfaces have been widely demonstrated in vitro but their use in animal models is not trivial. Here, we reported the interaction of mercaptosuccinic-coated (MSA) CdTe QDs with the epidermis of living and Carnoy-fixed zebrafish embryos. QDs concentrate along adherent junctions and reveal the characteristic pattern of actin microridges at the apical surface of the enveloping layer. In our study, labeling with anionic QDs is attained within few minutes at submicromolar concentrations in whole mounted Carnoy-fixed zebrafish embryos, providing a faster approach compared with immunodetection or standard Phalloidin staining of actin for visualization by fluorescence microscopy. Fil: Sigot, Valeria. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina Fil: Cabral Filho, Paulo E.. Universidade Federal de Pernambuco; Brasil Fil: Sampedro, Maria Florencia. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina Fil: Santos, Beate S.. Universidade Federal de Pernambuco; Brasil Fil: Fontes, Adriana. Universidade Federal de Pernambuco; Brasil
- Subjects :
- Phalloidin
02 engineering and technology
010402 general chemistry
01 natural sciences
purl.org/becyt/ford/1 [https]
chemistry.chemical_compound
Quantum Dots
Fluorescence microscope
Animals
General Materials Science
purl.org/becyt/ford/1.6 [https]
Instrumentation
Zebrafish
Spectroscopy
Actin
biology
Epidermis (botany)
Chemistry
ACTIN MICRORIDGES
technology, industry, and agriculture
equipment and supplies
021001 nanoscience & nanotechnology
biology.organism_classification
Atomic and Molecular Physics, and Optics
Actins
0104 chemical sciences
Staining
ANIONIC QUANTUM DOTS
Quantum dot
Biophysics
Surface modification
ZEBRAFISH EPIDERMIS
Epidermis
0210 nano-technology
Subjects
Details
- ISSN :
- 20506120
- Volume :
- 8
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Methods and applications in fluorescence
- Accession number :
- edsair.doi.dedup.....f357828c23d638091c83b091ca3cf001