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A qPCR expression assay of IFI44L gene differentiates viral from bacterial infections in febrile children
- Source :
- Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019), Scientific Reports, RUNA. Repositorio da Consellería de Sanidade e Sergas, Servizo Galego de Saúde (SERGAS)
- Publication Year :
- 2019
- Publisher :
- Nature Publishing Group, 2019.
-
Abstract
- The diagnosis of bacterial infections in hospital settings is currently performed using bacterial culture from sterile site, but they are lengthy and limited. Transcriptomic biomarkers are becoming promising tools for diagnosis with potential applicability in clinical settings. We evaluated a RT-qPCR assay for a 2-transcript host expression signature (FAM89A and IFI44L genes) inferred from microarray data that allow to differentiate between viral and bacterial infection in febrile children. This assay was able to discriminate viral from bacterial infections (P-value = 1.04 × 10−4; AUC = 92.2%; sensitivity = 90.9%; specificity = 85.7%) and showed very high reproducibility regardless of the reference gene(s) used to normalize the data. Unexpectedly, the monogenic IFI44L expression signature yielded better results than those obtained from the 2-transcript test (P-value = 3.59 × 10−5; AUC = 94.1%; sensitivity = 90.9%; specificity = 92.8%). We validated this IFI44L signature in previously published microarray and whole-transcriptome data from patients affected by different types of viral and bacterial infections, confirming that this gene alone differentiates between both groups, thus saving time, effort, and costs. Herein, we demonstrate that host expression microarray data can be successfully translated into a fast, highly accurate and relatively inexpensive in vitro assay that could be implemented in the clinical routine.
- Subjects :
- 0301 basic medicine
Male
Microbiological culture
Microarray
Gene Expression
lcsh:Medicine
Urine
Neisseria meningitidis
Transcriptome
0302 clinical medicine
Child
lcsh:Science
Multidisciplinary
gripe humana
meningitis
Bacterial Infections
Clinical routine
ARN
Virus Diseases
Child, Preschool
Female
Expression Signature
Biology
Real-Time Polymerase Chain Reaction
Seizures, Febrile
Article
orina
Diagnosis, Differential
03 medical and health sciences
expresión génica
Influenza, Human
Reference gene
Humans
RNA, Messenger
Transcriptomics
Gene
Microarray analysis techniques
ARN mensajero
Gene Expression Profiling
Tumor Suppressor Proteins
lcsh:R
Infant
transcriptoma
030104 developmental biology
Infeccións Bacterianas
Immunology
infecciones bacterianas
RNA
lcsh:Q
Gene expression
Bacterial infection
030217 neurology & neurosurgery
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....f354f66dd692d66f0b49a3a093956e75