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Expression of Cathepsin D in Primary and Metastatic Human Melanoma and Dysplastic Nevi
- Source :
- Journal of Investigative Dermatology. 104(3):340-344
- Publication Year :
- 1995
- Publisher :
- Elsevier BV, 1995.
-
Abstract
- High levels of cytosolic cathepsin D expression have been associated with poor prognosis in breast cancer node-negative patients. In this work, we provide evidence that three cell lines established from human metastatic melanomas-IIB-MEL-J, IIB-MEL-LES, and lIB-MEL-IAN-express high levels of procathepsin D mRNA. IIB-MEL-J cells secreted into the conditioned media about 30% of the newly synthesized protein, which was active at acidic pH. Melanoma tumors arising in nude mice after injection of the three different cell lines expressed high levels of procathepsin D mRNA, Moreover, 13 human metastatic melanomas expressed variable levels of procathepsin D mRNA. To study the possible association between cathepsin D expression and melanoma development, samples corresponding to 10 primary tumors, 11 metastatic melanomas, 10 dysplastic nevi, 27 nevocellular nevi, and normal melanocytes were studied by immunohistochemistry for cathepsin D-specific staining. We found that cathepsin D was expressed in all of the dysplastic nevi and primary and metastatic melanomas tested but in only 18% of nevocellular nevi (five of 27), whereas normal melanocytes showed no cathepsin D expression. The over- all data indicate that cathepsin D is expressed at a high level by melanoma cells, and because of its expression in preneoplastic lesions, it may be associated with melanoma development.
- Subjects :
- Pathology
medicine.medical_specialty
Cathepsin D
Gene Expression
Dermatology
Biology
Biochemistry
Breast cancer
Gene expression
medicine
Tumor Cells, Cultured
Humans
RNA, Messenger
neoplasms
Melanoma
Molecular Biology
Cathepsin
Messenger RNA
Cell Biology
medicine.disease
Immunohistochemistry
Cell culture
Culture Media, Conditioned
Cancer research
Dysplastic Nevus Syndrome
Subjects
Details
- ISSN :
- 0022202X
- Volume :
- 104
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of Investigative Dermatology
- Accession number :
- edsair.doi.dedup.....f35202f223049a9dbf118402a9b3029f
- Full Text :
- https://doi.org/10.1111/1523-1747.ep12665371