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First evidence of polar flagella in Klebsiella pneumoniae isolated from a patient with neonatal sepsis

First evidence of polar flagella in Klebsiella pneumoniae isolated from a patient with neonatal sepsis

Authors :
Miguel Castañeda-Lucio
Sirenia González-Posos
Carmen Torres
Patricia Lozano-Zarain
Claudia F. Martinez de la Peña
Libia León-Izurieta
Ygnacio Martínez-Laguna
Rosa del Carmen Rocha-Gracia
Zita Gutierrez-Cazarez
Alejandro Carabarin-Lima
Source :
RIUR. Repositorio Institucional de la Universidad de La Rioja, instname
Publication Year :
2016

Abstract

The genus Klebsiella belongs to the family Enterobacteriaceae, and is currently considered to be non-motile and non-flagellated. In the present work, 25 Klebsiella strains isolated from nosocomial infections were assessed for motility under different growth conditions. One Klebsiella isolate, KpBUAP021, demonstrated a swim-like motility phenotype. The K. pneumoniae genotype was confirmed by 16S rRNA and rpoB gene sequence analysis. Multilocus sequence typing analysis also revealed that the KpBUAP021 strain places it in the ST345 sequence type, and belongs to the phylogenetic Kpl group. Transmission electron microscopy and the Ryu staining technique revealed that KpBUAP021 expresses polar flagella. Finally, the presence of fliC, fliA and flgH genes in this K. pneumoniae strain was confirmed. This report presents the first evidence for flagella-mediated motility in a K. pneumoniae clinical isolate, and represents an important finding related to its evolution and pathogenic potential. © 2016 The Authors.

Details

Language :
English
Database :
OpenAIRE
Journal :
RIUR. Repositorio Institucional de la Universidad de La Rioja, instname
Accession number :
edsair.doi.dedup.....f35060561126e42450ea6653fd168af1