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First evidence of polar flagella in Klebsiella pneumoniae isolated from a patient with neonatal sepsis
First evidence of polar flagella in Klebsiella pneumoniae isolated from a patient with neonatal sepsis
- Source :
- RIUR. Repositorio Institucional de la Universidad de La Rioja, instname
- Publication Year :
- 2016
-
Abstract
- The genus Klebsiella belongs to the family Enterobacteriaceae, and is currently considered to be non-motile and non-flagellated. In the present work, 25 Klebsiella strains isolated from nosocomial infections were assessed for motility under different growth conditions. One Klebsiella isolate, KpBUAP021, demonstrated a swim-like motility phenotype. The K. pneumoniae genotype was confirmed by 16S rRNA and rpoB gene sequence analysis. Multilocus sequence typing analysis also revealed that the KpBUAP021 strain places it in the ST345 sequence type, and belongs to the phylogenetic Kpl group. Transmission electron microscopy and the Ryu staining technique revealed that KpBUAP021 expresses polar flagella. Finally, the presence of fliC, fliA and flgH genes in this K. pneumoniae strain was confirmed. This report presents the first evidence for flagella-mediated motility in a K. pneumoniae clinical isolate, and represents an important finding related to its evolution and pathogenic potential. © 2016 The Authors.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
DNA, Bacterial
Klebsiella
Klebsiella pneumoniae
Sequence analysis
030106 microbiology
Sigma Factor
Biology
Flagellum
Microbiology
DNA, Ribosomal
03 medical and health sciences
Bacterial Proteins
Microscopy, Electron, Transmission
RNA, Ribosomal, 16S
Genotype
Humans
Child
Phylogeny
Staining and Labeling
General Medicine
DNA-Directed RNA Polymerases
Sequence Analysis, DNA
Ribosomal RNA
biology.organism_classification
rpoB
Klebsiella Infections
030104 developmental biology
Flagella
Child, Preschool
Multilocus sequence typing
Neonatal Sepsis
Locomotion
Bacterial Outer Membrane Proteins
Flagellin
Multilocus Sequence Typing
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- RIUR. Repositorio Institucional de la Universidad de La Rioja, instname
- Accession number :
- edsair.doi.dedup.....f35060561126e42450ea6653fd168af1