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Modeling the presentation of C3d-coated antigen by B lymphocytes: enhancement by CR1/2–BCR co-ligation is selective for the co-ligating antigen
- Source :
- International Immunology. 14:241-247
- Publication Year :
- 2002
- Publisher :
- Oxford University Press (OUP), 2002.
-
Abstract
- We have used a set of single-chain variable fragment antibodies (sc) genetically fused with an influenza hemagglutinin-derived peptide as a means to investigate the role of CR1 and CR2 in antigen presentation by B cells. When incubated with the B cell lymphoma 2PK3, peptide-containing sc specific for either CR1 or CR1/2 mediated activation of the hemagglutinin peptide-specific T cell line IP-12-7, as assessed by IL-2 production. Efficient presentation was dependent on the binding of the constructs to CR1/2, implying that receptor-mediated endocytosis is responsible for the effect. Cross-linkage of CR1/2 or CD19 by mAb did not increase the extent of T cell activation. However, when CR1/2 was co-ligated with the BCR--using either polyclonal goat anti-mouse IgG or recombinant protein LA--the antigen concentration required to activate T cells decreased by two orders of magnitude. Moreover, this enhancement was selective for the antigen included in these complexes and did not affect the presentation of a free peptide or of antigen bound to CR1/2 excluded from the complexes. These results suggest that B cells may bind various C3d-coated antigens at a time, but only the one which reacts with the BCR will be processed with high efficiency. This mechanism may ensure the specificity of cognate T cell help.
- Subjects :
- Antigen Presentation
B-Lymphocytes
Antigen processing
Immunology
T-cell receptor
Antigen presentation
B-cell receptor
Naive B cell
Models, Immunological
Receptors, Antigen, B-Cell
General Medicine
Biology
Molecular biology
Receptors, Complement
Mice
Antigen
Complement C3d
Receptors, Complement 3b
Tumor Cells, Cultured
Animals
Immunology and Allergy
Cytotoxic T cell
Receptors, Complement 3d
Antigen-presenting cell
Cells, Cultured
Subjects
Details
- ISSN :
- 14602377 and 09538178
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- International Immunology
- Accession number :
- edsair.doi.dedup.....f34e1a7a193814006c0deeb879fab496