Back to Search
Start Over
Adenosine Receptor Stimulation Improves Glucocorticoid-Induced Osteoporosis in a Rat Model
- Source :
- Frontiers in Pharmacology, Frontiers in Pharmacology, Vol 8 (2017)
- Publication Year :
- 2017
- Publisher :
- Frontiers Media S.A., 2017.
-
Abstract
- Glucocorticoid-induced osteoporosis (GIO) is a secondary cause of bone loss. Bisphosphonates approved for GIO, might induce jaw osteonecrosis; thus additional therapeutics are required. Adenosine receptor agonists are positive regulators of bone remodeling, thus the efficacy of adenosine receptor stimulation for treating GIO was tested. In a preventive study GIO was induced in Sprague-Dawley rats by methylprednisolone (MP) for 60 days. Animals were randomly assigned to receive polydeoxyribonucleotide (PDRN), an adenosine A2 receptor agonist, or PDRN and DMPX (3,7-dimethyl-1-propargylxanthine, an A2 antagonist), or vehicle (0.9% NaCl). Another set of animals was used for a treatment study, following the 60 days of MP-induction rats were randomized to receive (for additional 60 days) PDRN, or PDRN and DMPX (an adenosine A2 receptor antagonist), or zoledronate (as control for gold standard treatment), or vehicle. Control animals were administered with vehicle for either 60 or 120 days. Femurs were analyzed after treatments for histology, imaging, and breaking strength analysis. MP treatment induced severe bone loss, the concomitant use of PDRN prevented the developing of osteoporosis. In rats treated for 120 days, PDRN restored bone architecture and bone strength; increased b-ALP, osteocalcin, osteoprotegerin and stimulated the Wnt canonical and non-canonical pathway. Zoledronate reduced bone resorption and ameliorated the histological features, without significant effects on bone formation. Our results suggest that adenosine receptor stimulation might be useful for preventing and treating GIO.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Osteoporosis
Bone resorption
PDRN
Bone remodeling
03 medical and health sciences
0302 clinical medicine
Osteoprotegerin
Internal medicine
Medicine
Pharmacology (medical)
Original Research
Pharmacology
glucocorticoids
business.industry
Adenosine, Glucocorticoids, Osteoporosis, PDRN, Rats, Pharmacology, Pharmacology (medical)
lcsh:RM1-950
medicine.disease
Adenosine
Adenosine receptor
osteoporosis
rats
030104 developmental biology
Endocrinology
lcsh:Therapeutics. Pharmacology
adenosine
DMPX
business
Osteonecrosis of the jaw
030217 neurology & neurosurgery
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 16639812
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Frontiers in Pharmacology
- Accession number :
- edsair.doi.dedup.....f34d2c963da996912b58a3a589a836ae