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Sphingosine-1-phosphate mobilizes osteoclast precursors and regulates bone homeostasis
- Source :
- Nature. 458:524-528
- Publication Year :
- 2009
- Publisher :
- Springer Science and Business Media LLC, 2009.
-
Abstract
- Osteoclasts are the only somatic cells with bone-resorbing capacity and, as such, they have a critical role not only in normal bone homeostasis (called 'bone remodelling') but also in the pathogenesis of bone destructive disorders such as rheumatoid arthritis and osteoporosis. A major focus of research in the field has been on gene regulation by osteoclastogenic cytokines such as receptor activator of NF-kappaB-ligand (RANKL, also known as TNFSF11) and TNF-alpha, both of which have been well documented to contribute to osteoclast terminal differentiation. A crucial process that has been less well studied is the trafficking of osteoclast precursors to and from the bone surface, where they undergo cell fusion to form the fully differentiated multinucleated cells that mediate bone resorption. Here we report that sphingosine-1-phosphate (S1P), a lipid mediator enriched in blood, induces chemotaxis and regulates the migration of osteoclast precursors not only in culture but also in vivo, contributing to the dynamic control of bone mineral homeostasis. Cells with the properties of osteoclast precursors express functional S1P(1) receptors and exhibit positive chemotaxis along an S1P gradient in vitro. Intravital two-photon imaging of bone tissues showed that a potent S1P(1) agonist, SEW2871, stimulated motility of osteoclast precursor-containing monocytoid populations in vivo. Osteoclast/monocyte (CD11b, also known as ITGAM) lineage-specific conditional S1P(1) knockout mice showed osteoporotic changes due to increased osteoclast attachment to the bone surface. Furthermore, treatment with the S1P(1) agonist FTY720 relieved ovariectomy-induced osteoporosis in mice by reducing the number of mature osteoclasts attached to the bone surface. Together, these data provide evidence that S1P controls the migratory behaviour of osteoclast precursors, dynamically regulating bone mineral homeostasis, and identifies a critical control point in osteoclastogenesis that may have potential as a therapeutic target.
- Subjects :
- musculoskeletal diseases
Bone density
Ovariectomy
Osteoclasts
Biology
Bone and Bones
Monocytes
Article
Bone resorption
Cell Line
Bone remodeling
Mice
Bone Density
Sphingosine
Osteoclast
medicine
Animals
Homeostasis
Cell Lineage
Bone Resorption
Bone mineral
Multidisciplinary
Fingolimod Hydrochloride
Chemotaxis
Cell biology
Mice, Inbred C57BL
Receptors, Lysosphingolipid
medicine.anatomical_structure
Positive chemotaxis
Propylene Glycols
RANKL
Immunology
biology.protein
Osteoporosis
Female
Lysophospholipids
Subjects
Details
- ISSN :
- 14764687 and 00280836
- Volume :
- 458
- Database :
- OpenAIRE
- Journal :
- Nature
- Accession number :
- edsair.doi.dedup.....f32e81894e421ff75e92cabffb8d4112