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Secretory leukocyte protease inhibitor inhibits expression of polymeric immunoglobulin receptor via the NF-κB signaling pathway
- Source :
- Molecular Immunology. 67(2):568-574
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Polymeric immunoglobulin receptor (pIgR) plays an important role in mucosal immune systems. Secretory immunoglobulin A, composed of secretory component of pIgR and a dimeric form of immunoglobulin A, is secreted on mucosal surfaces and serves as a biological defense factor. pIgR gene expression is reportedly induced by activation of the transcription factor nuclear factor (NF)-κB. On the other hand, secretory leukocyte protease inhibitor (SLPI) is a glycoprotein that functions as a serine protease inhibitor. In alveolar epithelial cells, SLPI increases the level of IκBβ, which indicates that it is an inhibitor of NF-κB at the protein level. Taken together, SLPI may regulate pIgR expression; however, the specific mechanism by which this occurs is unclear. Therefore, the aim of this study was to elucidatethe influence of SLPI on pIgR expression.SLPI and pIgR localized in goblet cells and ciliated epithelial cells of the gastrointestinal tract, respectively. No cells were detected in which SLPI and pIgR were co-expressed. In addition, recombinant human SLPI stimulation of an epithelial cell line (HT-29) decreased the pIgR expression. The pIgR expression was also higher in SLPI-deficient Ca9-22 cells than in wild-type Ca9-22 cells. Furthermore, a luciferase assay using a NF-κB reporter plasmid and real-time RT-PCR analysis indicated that when SLPI was present, the transcriptional activity of NF-κB protein was suppressed, which was accompanied by anincrease in the protein, but not the mRNA,expression of IκBβ. These results demonstrate that SLPI down-regulates pIgR expression through the NF-κB signaling pathway by inhibiting degradation of IκBβ protein.
- Subjects :
- Immunoglobulin A
Secretory component
Immunology
Salivary Glands
Gene Knockout Techniques
chemistry.chemical_compound
Humans
Secretory Leukocyte Peptidase Inhibitor
RNA, Messenger
Molecular Biology
Regulation of gene expression
biology
NF-kappa B
Receptors, Polymeric Immunoglobulin
NF-κB
NFKB1
Molecular biology
Recombinant Proteins
Secretory Component
Cell biology
Gastrointestinal Tract
Gene Expression Regulation
chemistry
Mutation
biology.protein
Signal transduction
Polymeric immunoglobulin receptor
HT29 Cells
Signal Transduction
SLPI
Subjects
Details
- ISSN :
- 01615890
- Volume :
- 67
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Molecular Immunology
- Accession number :
- edsair.doi.dedup.....f321f67637b6aae83bc9535e24ed4f85
- Full Text :
- https://doi.org/10.1016/j.molimm.2015.07.021