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Sugar chains of cerebrospinal fluid transferrin as a new biological marker of Alzheimer's disease
- Source :
- Dementia and geriatric cognitive disorders. 26(2)
- Publication Year :
- 2008
-
Abstract
- Background/Aims: Alzheimer’s disease (AD) is a well-known type of dementia. However, it remains difficult to identify AD in the early stage and to distinguish it from other dementing disorders. We examined glycoproteins in cerebrospinal fluid (CSF) as potential biological markers of AD. Methods: CSF samples were collected from AD, other dementia and nondemented patients. Glycoproteins in CSF were detected by lectin blotting using wheat germ agglutinin (WGA), and sugar chain analysis was performed by isoelectric focusing. Results: In Alzheimer’s CSF, several glycoproteins had lower WGA-binding activities, one of which was sufficiently sensitive and specific to distinguish AD from nondemented controls and other dementias. Further analysis identified this glycosylated protein as transferrin, and altered sugar chain composition of transferrin isoforms was observed despite normal protein levels in CSF. Conclusion: The decreased WGA-binding activity of transferrin in AD is probably due to altered glycosylation of transferrin molecules. Transferrin glycosylation is thus a potential biological marker for AD diagnosis, and changes in this glycosylation may play an important role in the pathophysiology of AD.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Glycosylation
Wheat Germ Agglutinins
Cognitive Neuroscience
tau Proteins
Disease
Central nervous system disease
Cerebrospinal fluid
Degenerative disease
Alzheimer Disease
medicine
Dementia
Humans
Phosphorylation
Aged
chemistry.chemical_classification
Aged, 80 and over
Amyloid beta-Peptides
Transferrin
medicine.disease
Peptide Fragments
Psychiatry and Mental health
chemistry
Tauopathies
Immunology
Female
Tauopathy
Geriatrics and Gerontology
Alzheimer's disease
Isoelectric Focusing
Psychology
Biomarkers
Subjects
Details
- ISSN :
- 14219824
- Volume :
- 26
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Dementia and geriatric cognitive disorders
- Accession number :
- edsair.doi.dedup.....f3025ae73e45e9109515ccafece68910