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Protective AntiâHelicobacterImmunity Is Induced with Aluminum Hydroxide or Complete Freund's Adjuvant by Systemic Immunization
- Source :
- The Journal of Infectious Diseases. 184:308-314
- Publication Year :
- 2001
- Publisher :
- Oxford University Press (OUP), 2001.
-
Abstract
- To determine whether systemic immunization against Helicobacter pylori could be achieved with an adjuvant approved for human use, the efficacy of vaccination with Helicobacter antigen in combination with aluminum hydroxide (AlOH) was evaluated in a murine model of Helicobacter infection. Immunization with antigen and AlOH induced interleukin-5-secreting, antigen-specific T cells, and immunization with antigen and complete Freund's adjuvant induced interferon-gamma-secreting, antigen-specific T cells, as determined by ELISPOT assay. Both immune responses conferred protection after challenge with either H. pylori or H. felis, as confirmed by the complete absence of any bacteria, as assessed by both histology and culture of gastric biopsy samples. Protection was antibody independent, as demonstrated with antibody-deficient muMT mice (immunoglobulin-gene knockout mice), and CD4(+) spleen T cells from immunized mice were sufficient to transfer protective immunity to otherwise immunodeficient rag1(-/-) recipients. These results suggest an alternative and potentially more expeditious strategy for development of a human-use H. pylori vaccine.
- Subjects :
- T-Lymphocytes
medicine.medical_treatment
Freund's Adjuvant
Aluminum Hydroxide
chemical and pharmacologic phenomena
Helicobacter Infections
Mice
Immune system
Antigen
Helicobacter
medicine
Animals
Humans
Immunology and Allergy
Antigens, Bacterial
B-Lymphocytes
Helicobacter pylori
biology
business.industry
ELISPOT
Vaccination
biology.organism_classification
Adoptive Transfer
Virology
Mice, Mutant Strains
Mice, Inbred C57BL
Infectious Diseases
Immunization
Gastric Mucosa
Freund's adjuvant
Immunology
biology.protein
Antibody
business
Adjuvant
Subjects
Details
- ISSN :
- 15376613 and 00221899
- Volume :
- 184
- Database :
- OpenAIRE
- Journal :
- The Journal of Infectious Diseases
- Accession number :
- edsair.doi.dedup.....f2f0eabc208fffe624dab747c40e6065