Back to Search
Start Over
Polydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healing
- Source :
- Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020), Scientific Reports
- Publication Year :
- 2020
- Publisher :
- Nature Portfolio, 2020.
-
Abstract
- Patients with diabetes experience delayed wound healing because of the uncontrolled glucose level in their bloodstream, which leads to impaired function of white blood cells, poor circulation, decreased production and repair of new blood vessels. Treatment using polydeoxyribonucleotide (PDRN), which is a DNA extracted from the sperm cells of salmon, has been introduced to accelerate the healing process of diabetic wounds. To accelerate the wound-healing process, sustained delivery of PDRN is critical. In this study, taking advantage of the non-invasive gelation property of alginate, PDRN was loaded inside the hydrogel (Alg-PDRN). The release behavior of PDRN was altered by controlling the crosslinking density of the Alg hydrogel. The amount of PDRN was the greatest inside the hydrogel with the highest crosslinking density because of the decreased diffusion. However, there was an optimal degree of crosslinking for the effective release of PDRN. In vitro studies using human dermal fibroblasts and diabetes mellitus fibroblasts and an in ovo chorioallantoic membrane assay confirmed that the Alg-PDRN hydrogel effectively induced cell proliferation and expression of angiogenic growth factors and promoted new blood vessel formation. Its effectiveness for accelerated diabetic wound healing was also confirmed in an in-vivo animal experiment using a diabetic mouse model.
- Subjects :
- Male
0301 basic medicine
Alginates
Science
Neovascularization, Physiologic
02 engineering and technology
Pharmacology
In ovo
Article
Neovascularization
Mice
03 medical and health sciences
Polydeoxyribonucleotides
Diabetes mellitus
Diabetes Mellitus
medicine
Animals
Humans
Wound Healing
Multidisciplinary
Cell growth
Health care
technology, industry, and agriculture
Hydrogels
Fibroblasts
021001 nanoscience & nanotechnology
medicine.disease
Materials science
In vitro
Mice, Inbred C57BL
Chorioallantoic membrane
030104 developmental biology
medicine.anatomical_structure
Diabetic wound healing
Medicine
medicine.symptom
0210 nano-technology
Blood vessel
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....f2e6aba69629dc233d9d3f200a04587d