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Regulation of Fas gene expression in HeLa cells as determined by modified RT-PCR
- Source :
- Cellular and Molecular Life Sciences CMLS. 54:186-190
- Publication Year :
- 1998
- Publisher :
- Springer Science and Business Media LLC, 1998.
-
Abstract
- We determined human Fas messenger RNA (mRNA) levels in HeLa cells using a 'mutagenic' reverse transcription-polymerase chain reaction, which quantitates mRNA levels using the corresponding genomic DNA as an internal control. The expression level of Fas mRNA was very low in serum-deprived quiescent HeLa cells. In conjunction with the start of cell-cycle progression upon the addition of serum to culture medium, the Fas mRNA level gradually increased, reached its peak at 36 h and returned to the basal level after 48 h. HeLa cells at 36 h exhibiting a high level of Fas mRNA expression were more susceptible to the anti-Fas antibody apoptotic signal. Thus, the regulation of Fas expression is associated with cell-cycle progression, and this method for Fas mRNA detection may be useful, particularly for the analysis of small amounts of samples.
- Subjects :
- Apoptosis
Polymerase Chain Reaction
Antibodies
HeLa
Cellular and Molecular Neuroscience
Humans
RNA, Messenger
fas Receptor
Molecular Biology
Pharmacology
Regulation of gene expression
Messenger RNA
biology
Cell Cycle
RNA
Cell Biology
Cell cycle
biology.organism_classification
Fas receptor
Molecular biology
Culture Media
Cell biology
Gene Expression Regulation, Neoplastic
Real-time polymerase chain reaction
Molecular Medicine
HeLa Cells
Subjects
Details
- ISSN :
- 14209071 and 1420682X
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- Cellular and Molecular Life Sciences CMLS
- Accession number :
- edsair.doi.dedup.....f2d7914e00de29f0f724297fb7f4f16c