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Analysis of ELA-DQB exon 2 polymorphism in Argentine Creole horses by PCR-RFLP and PCR-SSCP
- Source :
- Journal of veterinary medicine. A, Physiology, pathology, clinical medicine. 50(6)
- Publication Year :
- 2003
-
Abstract
- The second exon of equine leucocyte antigen (ELA)-DQB genes was amplified from genomic DNA of 32 Argentine Creole horses by PCR. Amplified DNA was analysed by PCR-restriction fragment length polymorphism (RFLP) and PCR-single-strand conformation polymorphism (SSCP). The PCR-RFLP analysis revealed two HaeIII patterns, four RsaI patterns, five MspI patterns and two HinfI patterns. EcoRI showed no variation in the analysed sample. Additional patterns that did not account for known exon 2 DNA sequences were observed, suggesting the existence of novel ELA-DQB alleles. PCR-SSCP analysis exhibited seven different band patterns, and the number of bands per animal ranged from four to nine. Both methods indicated that at least two DQB genes are present. The presence of more than two alleles in each animal showed that the primers employed in this work are not specific for a unique DQB locus. The improvement of this PCR-RFLP method should provide a simple and rapid technique for an accurate definition of ELA-DQB typing in horses.
- Subjects :
- Restriction Mapping
Argentina
Locus (genetics)
Biology
Breeding
Polymerase Chain Reaction
DNA sequencing
Pathology and Forensic Medicine
HaeIII
Exon
medicine
Animals
Typing
Horses
Phylogeny
Polymorphism, Single-Stranded Conformational
DNA Primers
Genetics
Polymorphism, Genetic
General Veterinary
Histocompatibility Antigens Class II
Genetic Variation
Single-strand conformation polymorphism
Exons
Molecular biology
genomic DNA
Restriction fragment length polymorphism
Polymorphism, Restriction Fragment Length
medicine.drug
Subjects
Details
- ISSN :
- 0931184X
- Volume :
- 50
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Journal of veterinary medicine. A, Physiology, pathology, clinical medicine
- Accession number :
- edsair.doi.dedup.....f2d69aa52ef5a741748a272d69185d92