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A novel moderately thermophilic facultative methylotroph within the class Alphaproteobacteria
- Source :
- Microorganisms, Microorganisms, Vol 9, Iss 477, p 477 (2021), Volume 9, Issue 3
- Publication Year :
- 2021
-
Abstract
- Methylotrophic bacteria (non-methanotrophic methanol oxidizers) consuming reduced carbon compounds containing no carbon–carbon bonds as their sole carbon and energy source have been found in a great variety of environments. Here, we report a unique moderately thermophilic methanol-oxidising bacterium (strain LS7-MT) that grows optimally at 55 °C (with a growth range spanning 30 to 60 °C). The pure isolate was recovered from a methane-utilizing mixed culture enrichment from an alkaline thermal spring in the Ethiopia Rift Valley, and utilized methanol, methylamine, glucose and a variety of multi-carbon compounds. Phylogenetic analysis of the 16S rRNA gene sequences demonstrated that strain LS7-MT represented a new facultatively methylotrophic bacterium within the order Hyphomicrobiales of the class Alphaproteobacteria. This new strain showed 94 to 96% 16S rRNA gene identity to the two methylotroph genera, Methyloceanibacter and Methyloligella. Analysis of the draft genome of strain LS7-MT revealed genes for methanol dehydrogenase, essential for methanol oxidation. Functional and comparative genomics of this new isolate revealed genomic and physiological divergence from extant methylotrophs. Strain LS7-MT contained a complete mxaF gene cluster and xoxF1 encoding the lanthanide-dependent methanol dehydrogenase (XoxF). This is the first report of methanol oxidation at 55 °C by a moderately thermophilic bacterium within the class Alphaproteobacteria. These findings expand our knowledge of methylotrophy by the phylum Proteobacteria in thermal ecosystems and their contribution to global carbon and nitrogen cycles.
- Subjects :
- Microbiology (medical)
Alphaproteobacteria
methanol oxidation
Microbiology
03 medical and health sciences
Virology
Gene cluster
Botany
lcsh:QH301-705.5
030304 developmental biology
0303 health sciences
biology
Methanol dehydrogenase
Strain (chemistry)
030306 microbiology
Chemistry
Thermophile
biology.organism_classification
16S ribosomal RNA
facultative
thermal spring
lcsh:Biology (General)
Methylotroph
Energy source
moderately thermophilic
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Microorganisms, Microorganisms, Vol 9, Iss 477, p 477 (2021), Volume 9, Issue 3
- Accession number :
- edsair.doi.dedup.....f2c8f5186a08742d138ed5cf6a394f88