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Changes in Pain Behavior and Histologic Changes Caused by Application of Tumor Necrosis Factor-Alpha to the Dorsal Root Ganglion in Rats
- Source :
- Spine. 31:530-535
- Publication Year :
- 2006
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2006.
-
Abstract
- Study design Histologic changes in the dorsal root ganglion (DRG) and the nociceptive stimulation thresholds were studied in rats. Objective To examine the effects of tumor necrosis factor-alpha (TNF) with special reference to pain behavior and histology of the DRG. Summary of background data Recently, it was reported that local application of nucleus pulposus induces a characteristic tissue reaction at the surface of the DRG. However, to our knowledge, there have been no previous reports about the relationship between the histologic changes and pain behavior caused by cytokines. Methods Recombinant TNF was applied to the L4 DRG. Mechanical and thermal nociceptive thresholds were tested. The L4 DRG was sectioned and observed by light microscopy. Results After the application of 5 ng/microL TNF, significant differences were observed in mechanical and thermal stimulation thresholds. At the site of application of TNF, a characteristic a semilunar-shaped enlargement was observed. The average width of the part was significantly larger in the 5 ng/microL TNF application, as compared to the 0.5-ng/microL TNF application. Conclusions The higher concentration of TNF used induced allodynia and hyperalgesia responses. Because the region showing the histologic changes was significantly larger after application of the higher concentration of TNF, the reaction of the DRG may be related to pain.
- Subjects :
- Pain Threshold
Pathology
medicine.medical_specialty
Hot Temperature
Administration, Topical
medicine.medical_treatment
Pain
Stimulation
Zygapophyseal Joint
Rats, Sprague-Dawley
Dorsal root ganglion
Ganglia, Spinal
Physical Stimulation
medicine
Animals
Orthopedics and Sports Medicine
Lumbar Vertebrae
Behavior, Animal
Dose-Response Relationship, Drug
Tumor Necrosis Factor-alpha
business.industry
Spinal cord
Recombinant Proteins
Rats
Allodynia
Cytokine
medicine.anatomical_structure
Nociception
Hyperalgesia
Touch
Sensory Thresholds
Anesthesia
Female
Tumor necrosis factor alpha
Neurology (clinical)
medicine.symptom
business
Subjects
Details
- ISSN :
- 03622436
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Spine
- Accession number :
- edsair.doi.dedup.....f2aaddf77b2bb424d75642fcea0923a3