Back to Search
Start Over
Studies in red blood cell preservation. 2. Comparison of vesicle formation, morphology, and membrane lipids during storage in AS-1 and CPDA-1
- Source :
- Vox sanguinis. 58(2)
- Publication Year :
- 1990
-
Abstract
- The changes in morphology, the quantitative changes in membrane lipids and the shedding of exocytic vesicles by red blood cells (RBC) stored for 42 and 56 days in AS-1 and CPDA-1 were compared. RBC stored in AS-1 shed significantly less vesicle membrane cholesterol, phospholipid and protein and maintained better morphology scores. RBC membrane cholesterol remained higher after 56 days in AS-1 than in CPDA-1. The data suggest that during the first weeks of storage cholesterol is lost from the RBC membrane followed by a larger release of phospholipids accompanied by alterations in the phosphoinositides. The shedding of exocytic vesicles appears to be secondary to the changes in morphology resulting from the perturbation of the membrane lipids.
- Subjects :
- Erythrocytes
Sodium
Membrane lipids
Phospholipid
chemistry.chemical_element
Biology
In Vitro Techniques
Sodium Chloride
Phosphates
chemistry.chemical_compound
medicine
Humans
Mannitol
Citrates
Phospholipids
Cholesterol
Vesicle
Adenine
Erythrocyte Membrane
Membrane Proteins
Hematology
General Medicine
Cell biology
Red blood cell
medicine.anatomical_structure
Glucose
chemistry
Membrane protein
Blood Preservation
lipids (amino acids, peptides, and proteins)
medicine.drug
Subjects
Details
- ISSN :
- 00429007
- Volume :
- 58
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Vox sanguinis
- Accession number :
- edsair.doi.dedup.....f29741fef8f3418f8ea40222d5220d3f