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Optimization of the purine operon and energy generation in Bacillus amyloliquefaciens for guanosine production
- Source :
- Biotechnology Letters. 39:1675-1682
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- To deregulate the purine operon of the purine biosynthetic pathway and optimize energy generation of the respiratory chain to improve the yield of guanosine in Bacillus amyloliquefaciens XH7. The 5′-untranslated region of the purine operon, which contains the guanine-sensing riboswitch, was disrupted. The native promoter Pw in B. amyloliquefaciens XH7 was replaced by different strong promoters. Among the promoter replacement mutants, XH7purE::P41 gave the highest guanosine yield (16.3 g/l), with an increase of 23% compared with B. amyloliquefaciens XH7. The relative expression levels of the purine operon genes (purE, purF, and purD) in the XH7purE::P41 mutant were upregulated. The concentration of inosine monophosphate (IMP), the primary intermediate in the purine pathway, was also significantly increased in the XH7purE::P41 mutant. Combined modification of the low-coupling branched respiratory chains (cytochrome bd oxidase) improved guanosine production synergistically. The final guanosine yield in the XH7purE::P41△cyd mutant increased by 41% to 19 g/l compared with B. amyloliquefaciens XH7. The combined modification strategy used in this study is a novel approach to improve the production of guanosine in industrial bacterial strains.
- Subjects :
- 0301 basic medicine
Inosine monophosphate
Purine
Bacillus amyloliquefaciens
Operon
Mutant
Respiratory chain
Guanosine
Bioengineering
Biology
Applied Microbiology and Biotechnology
03 medical and health sciences
chemistry.chemical_compound
Cloning, Molecular
Promoter Regions, Genetic
Purine Nucleotides
Oxidase test
General Medicine
biology.organism_classification
Biosynthetic Pathways
030104 developmental biology
Biochemistry
chemistry
5' Untranslated Regions
Energy Metabolism
Biotechnology
Subjects
Details
- ISSN :
- 15736776 and 01415492
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Biotechnology Letters
- Accession number :
- edsair.doi.dedup.....f27f53cfb650144680a174c98213a70d