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Medial prefrontal cortex endocannabinoid system modulates baroreflex activity through CB1receptors
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Publication Year :
- 2012
- Publisher :
- American Physiological Society, 2012.
-
Abstract
- Neural reflex mechanisms, such as the baroreflex, are involved in the regulation of cardiovascular system activity. Previous results from our group (Resstel LB, Correa FM. Medial prefrontal cortex NMDA receptors and nitric oxide modulate the parasympathetic component of the baroreflex. Eur J Neurosci 23: 481–488, 2006) have shown that glutamatergic synapses in the ventral portion of the medial prefrontal cortex (vMPFC) modulate baroreflex activity. Moreover, glutamatergic neurotransmission in the vMPFC can be modulated by the endocannabinoids system (eCBs), particularly the endocannabinoid anandamide, through presynaptic CB1receptor activation. Therefore, in the present study, we investigated eCBs receptors that are present in the vMPFC, and more specifically whether CB1receptors modulate baroreflex activity. We found that bilateral microinjection of the CB1receptor antagonist AM251 (100 or 300 pmol/200 nl) into the vMPFC increased baroreflex activity in unanesthetized rats. Moreover, bilateral microinjection of either the anandamide transporter inhibitor AM404 (100 pmol/200 nl) or the inhibitor of the enzyme fatty acid amide hydrolase that degrades anandamide, URB597 (100 pmol/200 nl), into the MPFC decreased baroreflex activity. Finally, pretreatment of the vMPFC with an ineffective dose of AM251 (10 pmol/200 nl) was able to block baroreflex effects of both AM404 and URB597. Taken together, our results support the view that the eCBs in the vMPFC is involved in the modulation of baroreflex activity through the activation of CB1receptors, which modulate local glutamate release.
- Subjects :
- Male
medicine.medical_specialty
Cannabinoid receptor
Physiology
Infralimbic cortex
Glutamic Acid
Prefrontal Cortex
Blood Pressure
CÓRTEX PRÉ-FRONTAL (FISIOLOGIA)
Arachidonic Acids
Baroreflex
Amidohydrolases
chemistry.chemical_compound
Piperidines
Receptor, Cannabinoid, CB1
Physiology (medical)
Internal medicine
Cannabinoid Receptor Modulators
medicine
Animals
Rats, Wistar
Receptor
Prefrontal cortex
Chemistry
musculoskeletal, neural, and ocular physiology
Anandamide
Endocannabinoid system
Rats
medicine.anatomical_structure
Endocrinology
nervous system
Benzamides
Reflex
Pyrazoles
Carbamates
Neuroscience
Endocannabinoids
Subjects
Details
- ISSN :
- 15221490 and 03636119
- Volume :
- 302
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
- Accession number :
- edsair.doi.dedup.....f267daacb49960fe25c1030102a1744d