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Expression of adhesion molecules by human endothelial cells exposed to oxidized low density lipoprotein Influences of degree of oxidation and location of oxidized LDL
- Source :
- Atherosclerosis. 154:79-86
- Publication Year :
- 2001
- Publisher :
- Elsevier BV, 2001.
-
Abstract
- The main objective of this study was to determine the influence of the degree of low density lipoprotein (LDL) oxidation and the location of oxidized LDL (oxLDL) on expression of adhesion molecules on endothelial cells (EC). OxLDL preparations 1-4 with different degrees of oxidative modification were studied. All preparations of oxLDL, after addition to the medium, stimulated the expression of intercellular adhesion molecule-1 (ICAM-1) by human umbilical vein endothelial cells (HUVEC) as determined by cell-ELISA. Concentration-dependent studies examining ICAM-1 expression by HUVEC showed that the minimal concentration of oxLDL which significantly stimulated ICAM-1 expression was 5 microg/ml, suggesting that the predicted physiological concentration of oxLDL in plasma may be not high enough to elicit a substantial increase of ICAM-1 expression in EC. In contrast, very small amounts (0.15 microg/well) of oxLDL-3 and 4, the more heavily oxidized LDL preparations, stimulated effectively ICAM-1 expression by HUVEC when located below the endothelial cell monolayer by immobilizing to type I collagen. The results suggest that the increased expression of ICAM-1 induced by accumulated oxLDL may be one of the mechanisms by which oxLDL contributes to atherogenesis.
- Subjects :
- Endothelium
Intercellular Adhesion Molecule-1
Vascular Cell Adhesion Molecule-1
Umbilical vein
chemistry.chemical_compound
medicine
Humans
Protein Isoforms
Tissue Distribution
Cells, Cultured
Dose-Response Relationship, Drug
Cell adhesion molecule
Osmolar Concentration
Adhesion
Molecular biology
Lipoproteins, LDL
Endothelial stem cell
medicine.anatomical_structure
chemistry
Biochemistry
Low-density lipoprotein
Cytokines
lipids (amino acids, peptides, and proteins)
Collagen
Endothelium, Vascular
E-Selectin
Cardiology and Cardiovascular Medicine
Oxidation-Reduction
Type I collagen
Subjects
Details
- ISSN :
- 00219150
- Volume :
- 154
- Database :
- OpenAIRE
- Journal :
- Atherosclerosis
- Accession number :
- edsair.doi.dedup.....f25e34a6ef3d549ee4ea3784504c80d8