Back to Search
Start Over
Collagen promotes matrix vesicle-mediated mineralization by vascular smooth muscle cells
- Source :
- Journal of Inorganic Biochemistry, Journal of Inorganic Biochemistry, Elsevier, 2018, 186, pp.1-9. ⟨10.1016/j.jinorgbio.2018.05.007⟩
- Publication Year :
- 2018
-
Abstract
- Vascular calcification (VC) is a hallmark of atherosclerotic plaques. Calcification of advanced plaques shares common features with endochondral ossification of long bones and appears to be protective. On the other hand, microcalcification of early plaques, which is poorly understood, is thought to be harmful. Tissue-nonspecific alkaline phosphatase (TNAP) and collagen are the two proteins necessary for physiological mineralization. Here, we demonstrate the presence of membrane-bound TNAP, detected by immunofluorescence, that seems to form clusters on the plasma membrane of vascular smooth muscle cells (VSMCs) cultured in mineralizing conditions. We observed that TNAP activity and mineralization were increased when VSMCs were cultured in the presence of ascorbic acid (AA) and β-glycerophosphate (β-GP). Increased TNAP activity was observed in whole cell lysates, total membrane fractions and, more particularly, in matrix vesicles (MVs). We have shown that TNAP-enriched MVs released from VSMCs subjected to collagenase contained more apatite-like mineral than the less TNAP-rich/TNAP-enriched vesicles isolated without collagenase treatment. These results suggest a role for collagen in promoting calcification induced by TNAP in atherosclerotic plaques.
- Subjects :
- 0301 basic medicine
Vascular smooth muscle
Myocytes, Smooth Muscle
030204 cardiovascular system & hematology
Biochemistry
Mineralization (biology)
Muscle, Smooth, Vascular
Inorganic Chemistry
03 medical and health sciences
Mice
0302 clinical medicine
medicine
Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Vascular Calcification
Endochondral ossification
ComputingMilieux_MISCELLANEOUS
Chemistry
Vesicle
medicine.disease
Ascorbic acid
Alkaline Phosphatase
Atherosclerosis
3. Good health
Cell biology
030104 developmental biology
Collagenase
Alkaline phosphatase
Collagen
Calcification
medicine.drug
Subjects
Details
- ISSN :
- 18733344 and 01620134
- Volume :
- 186
- Database :
- OpenAIRE
- Journal :
- Journal of inorganic biochemistry
- Accession number :
- edsair.doi.dedup.....f25c9714e4a53a19e8a3322d9ff35b10