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Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy
- Source :
- PLoS ONE, Vol 14, Iss 8, p e0221296 (2019), PLoS ONE
- Publication Year :
- 2019
- Publisher :
- Public Library of Science (PLoS), 2019.
-
Abstract
- Nanometre-scale observation of specimens in water is indispensable in several scientific fields, such as biology, chemistry, materials science and nanotechnology. Scanning electron microscopy (SEM) obtains high-resolution images of biological samples under high vacuum conditions but requires specific sample-preparation protocols. Observations of unstained biological samples in water require more convenient and less invasive methods. Herein, we have developed a new type of impedance microscopy, namely impedance SEM (IP-SEM), which allows the imaging and sub-micrometer scale examination of various specimens in water. By varying the frequency of the input signal, the proposed system can detect the impedance properties of the sample's composition at sub-micrometer scale resolution. Besides examining various unstained biological specimens and material samples in water. Furthermore, the proposed system can be used for diverse liquid samples across a broad range of scientific fields, such as nanoparticles, nanotubes and organic and catalytic materials.
- Subjects :
- 0301 basic medicine
Statistical methods
Scanning electron microscope
Polymers
Physiology
Nanoparticle
02 engineering and technology
Microscopy
Medicine and Health Sciences
Electric Impedance
Nanotechnology
Electron Microscopy
Materials
Multidisciplinary
Nanotubes
Physics
Resolution (electron density)
Statistics
Classical Mechanics
Body Fluids
Monte Carlo method
Chemistry
Milk
Macromolecules
Physical Sciences
Medicine
Bubbles
Scanning Electron Microscopy
Anatomy
Research Article
Chemical Elements
Materials science
Sample (material)
Science
0206 medical engineering
Ultra-high vacuum
Materials Science
Fluid Mechanics
Research and Analysis Methods
Continuum Mechanics
Resonance
Tungsten
Specimen Handling
Beverages
03 medical and health sciences
Biological specimen
Electrical impedance
Nutrition
Biology and Life Sciences
Water
Fluid Dynamics
Insulators
Polymer Chemistry
020601 biomedical engineering
Diet
Resonance Frequency
030104 developmental biology
Acrylics
Microscopy, Electron, Scanning
Mathematical and statistical techniques
Feasibility Studies
Nanoparticles
Dielectrics
Mathematics
Biomedical engineering
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 14
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....f253dd6f66ba8c7cfe3a4bd9d1718976