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Racemization in cataractous lens from diabetic and aging individuals: analysis of Asp 58 residue in αA-crystallin
- Source :
- Aging (Albany NY)
- Publication Year :
- 2021
- Publisher :
- Impact Journals, 2021.
-
Abstract
- Cataract is the leading cause of visual impairment globally. Racemization of lens proteins may contribute to cataract formation in aging individuals. As a special type of age-related cataract (ARC), diabetic cataract (DC) is characterized by the early onset of cortical opacification and finally developed into a mixed type of cortical and nuclear opacification. We compared racemization of Asp 58 residue, a hotspot position in αA-crystallin, from the cortex and nucleus of diabetic and age-matched senile cataractous lenses, by identifying L-Asp/L-isoAsp/D-Asp/D-isoAsp by mass spectrometry. Compared to nondiabetic cataractous lenses, DC lenses showed a significantly increased cortex/nucleus ratio of D-Asp 58, which originated primarily from an increased percentage of D-Asp 58 in the lens cortex of DC. Moreover, patients diagnosed with diabetes for over 10 years showed a lower cortex/nucleus ratio of D-isoAsp 58 in the lens compared with those who had a shorter duration of diabetes, which originated mainly from an increased percentage of D-isoAsp 58 in the lens nucleus of DC with increasing time of hyperglycemia. Further analysis confirmed decreased protein solubility in diabetic cataractous lenses. The different racemization pattern in DC may be distinguished from ARC and influence its phenotype over the protracted duration of diabetes.
- Subjects :
- Male
medicine.medical_specialty
Aging
genetic structures
lens
Cataract
Lens protein
Isomerism
Crystallin
Cortex (anatomy)
Internal medicine
Diabetes mellitus
crystallin
Lens, Crystalline
medicine
Humans
aspartyl residue
Racemization
Aged
Cell Nucleus
Aspartic Acid
Arc (protein)
diabetes
Chemistry
Cell Biology
medicine.disease
Crystallins
eye diseases
medicine.anatomical_structure
Endocrinology
Solubility
racemization
Cataractous lens
Female
sense organs
Nucleus
Research Paper
Subjects
Details
- Language :
- English
- ISSN :
- 19454589
- Volume :
- 13
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Aging (Albany NY)
- Accession number :
- edsair.doi.dedup.....f245f945eb331d9a5eac319128a0a870