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ESTs from Seeds to Assist the Selective Breeding of Jatropha curcas L. for Oil and Active Compounds
- Source :
- Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice), Empresa Brasileira de Pesquisa Agropecuária (Embrapa), instacron:EMBRAPA, Genomics Insights
- Publication Year :
- 2010
- Publisher :
- SAGE Publications, 2010.
-
Abstract
- We report here on the characterization of a cDNA library from seeds of Jatropha curcas L. at three stages of fruit maturation before yellowing. We sequenced a total of 2200 clones and obtained a set of 931 non-redundant sequences (unigenes) after trimming and quality control, ie, 140 contigs and 791 singlets with PHRED quality $10. We found low levels of sequence redundancy and extensive metabolic coverage by homology comparison to GO. After comparison of 5841 non-redundant ESTs from a total of 13193 reads from GenBank with KEGG, we identified tags with nucleotide variations among J. curcas accessions for genes of fatty acid, terpene, alkaloid, quinone and hormone pathways of biosynthesis. More specifically, the expression level of four genes (palmitoyl-acyl carrier protein thioesterase, 3-ketoacyl-CoA thiolase B, lysophosphatidic acid acyltransferase and geranyl pyrophosphate synthase) measured by real- time PCR proved to be significantly different between leaves and fruits. Since the nucleotide polymorphism of these tags is associated to higher level of gene expression in fruits compared to leaves, we propose this approach to speed up the search for quantitative traits in selective breeding of J. curcas. We also discuss its potential utility for the selective breeding of economically important traits in J. curcas. Diponível em:.Acesso em: 03 nov. 2010.
- Subjects :
- Biocombustível
alkaloids
Selective breeding
fatty acids
Biochemistry
chemistry.chemical_compound
Biofuel
Jatropha curcas
Complementary DNA
genomics
Genetics
Pinhão manso
Molecular Biology
Gene
Original Research
Expressed sequence tag
biology
Terpenes
business.industry
Geranyl pyrophosphate
Jatropha Curcas
Cell Biology
biology.organism_classification
Terpenoid
Biotechnology
chemistry
Melhoramento genetico vegatal
GenBank
biofuel
business
terpenes
Subjects
Details
- ISSN :
- 11786310
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- Genomics Insights
- Accession number :
- edsair.doi.dedup.....f242205c0b488326624e5e83e08c6d9f