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ESTs from Seeds to Assist the Selective Breeding of Jatropha curcas L. for Oil and Active Compounds

Authors :
Ivon Pinheiro Lôbo
Ana Carolina Ramos Guimarães
Tiago C. Almeida
J.C.M. Cascardo
Abelmon da Silva Gesteira
Rosenira Serpa da Cruz
Antonio Basílio de Miranda
Nicolas Carels
Kleber Alves Gomes
Marie-Anne Van Sluys
Kleber A. Gomes, UESC
Tiago C. Almeida, UESC
ABELMON DA SILVA GESTEIRA, CNPMF
Ivon Pinheiro Lobo, UESC
Ana Carolina R. Guimarães, FIOCRUZ
Antonio B. de Miranda, FIOCRUZ
Marie-Anne Van Sluys, USP
Rosenira Serpa da Cruz, UESC
Júlio Cézar Mattos Cascardo, UESC
Nicolas Carels, UESC.
Source :
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice), Empresa Brasileira de Pesquisa Agropecuária (Embrapa), instacron:EMBRAPA, Genomics Insights
Publication Year :
2010
Publisher :
SAGE Publications, 2010.

Abstract

We report here on the characterization of a cDNA library from seeds of Jatropha curcas L. at three stages of fruit maturation before yellowing. We sequenced a total of 2200 clones and obtained a set of 931 non-redundant sequences (unigenes) after trimming and quality control, ie, 140 contigs and 791 singlets with PHRED quality $10. We found low levels of sequence redundancy and extensive metabolic coverage by homology comparison to GO. After comparison of 5841 non-redundant ESTs from a total of 13193 reads from GenBank with KEGG, we identified tags with nucleotide variations among J. curcas accessions for genes of fatty acid, terpene, alkaloid, quinone and hormone pathways of biosynthesis. More specifically, the expression level of four genes (palmitoyl-acyl carrier protein thioesterase, 3-ketoacyl-CoA thiolase B, lysophosphatidic acid acyltransferase and geranyl pyrophosphate synthase) measured by real- time PCR proved to be significantly different between leaves and fruits. Since the nucleotide polymorphism of these tags is associated to higher level of gene expression in fruits compared to leaves, we propose this approach to speed up the search for quantitative traits in selective breeding of J. curcas. We also discuss its potential utility for the selective breeding of economically important traits in J. curcas. Diponível em:.Acesso em: 03 nov. 2010.

Details

ISSN :
11786310
Volume :
3
Database :
OpenAIRE
Journal :
Genomics Insights
Accession number :
edsair.doi.dedup.....f242205c0b488326624e5e83e08c6d9f