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Altered Gut Microbiota and Its Metabolites in Hypertension of Developmental Origins: Exploring Differences between Fructose and Antibiotics Exposure
- Source :
- International Journal of Molecular Sciences, Vol 22, Iss 2674, p 2674 (2021), International Journal of Molecular Sciences, Volume 22, Issue 5
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Gut microbiota-derived metabolites, in particular short chain fatty acids (SCFAs) and their receptors, are linked to hypertension. Fructose and antibiotics are commonly used worldwide, and they have a negative impact on the gut microbiota. Our previous study revealed that maternal high-fructose (HF) diet-induced hypertension in adult offspring is relevant to altered gut microbiome and its metabolites. We, therefore, intended to examine whether minocycline administration during pregnancy and lactation may further affect blood pressure (BP) programmed by maternal HF intake via mediating gut microbiota and SCFAs. Pregnant Sprague-Dawley rats received a normal diet or diet containing 60% fructose throughout pregnancy and lactation periods. Additionally, pregnant dams received minocycline (50 mg/kg/day) via oral gavage or a vehicle during pregnancy and lactation periods. Four groups of male offspring were studied (n = 8 per group): normal diet (ND), high-fructose diet (HF), normal diet + minocycline (NDM), and HF + minocycline (HFM). Male offspring were killed at 12 weeks of age. We observed that the HF diet and minocycline administration, both individually and together, causes the elevation of BP in adult male offspring, while there is no synergistic effect between them. Four groups displayed distinct enterotypes. Minocycline treatment leads to an increase in the F/B ratio, but decreased abundance of genera Lactobacillus, Ruminococcus, and Odoribacter. Additionally, minocycline treatment decreases plasma acetic acid and butyric acid levels. Hypertension programmed by maternal HF diet plus minocycline exposure is related to the increased expression of several SCFA receptors. Moreover, minocycline- and HF-induced hypertension, individually or together, is associated with the aberrant activation of the renin–angiotensin system (RAS). Conclusively, our results provide a new insight into the support of gut microbiota and its metabolite SCAFs in the developmental programming of hypertension and cast new light on the role of RAS in this process, which will help prevent hypertension programmed by maternal high-fructose and antibiotic exposure.
- Subjects :
- 0301 basic medicine
Male
Metabolite
030204 cardiovascular system & hematology
Gut flora
Kidney
fructose
Rats, Sprague-Dawley
Renin-Angiotensin System
lcsh:Chemistry
chemistry.chemical_compound
0302 clinical medicine
minocycline
Pregnancy
Lactobacillus
Lactation
renin–angiotensin system
lcsh:QH301-705.5
Spectroscopy
biology
General Medicine
Minocycline
Computer Science Applications
Anti-Bacterial Agents
medicine.anatomical_structure
Prenatal Exposure Delayed Effects
Female
medicine.drug
medicine.medical_specialty
hypertension
Normal diet
Offspring
short chain fatty acid
developmental origins of health and disease (DOHaD)
Receptors, Cell Surface
Gram-Positive Bacteria
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
nitric oxide
Internal medicine
Gram-Negative Bacteria
medicine
Animals
Physical and Theoretical Chemistry
Molecular Biology
gut microbiota
business.industry
Organic Chemistry
biology.organism_classification
medicine.disease
Fatty Acids, Volatile
Gastrointestinal Microbiome
Rats
030104 developmental biology
Endocrinology
chemistry
lcsh:Biology (General)
lcsh:QD1-999
business
Subjects
Details
- Language :
- English
- ISSN :
- 16616596 and 14220067
- Volume :
- 22
- Issue :
- 2674
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....f2226ba31afdd80270a50c9f0ce93ce7