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PRR14 overexpression promotes cell growth, epithelial to mesenchymal transition and metastasis of colon cancer via the AKT pathway
- Source :
- PLoS ONE, PLoS ONE, Vol 14, Iss 10, p e0218839 (2019)
- Publication Year :
- 2019
-
Abstract
- BackgroundPRR14 (Proline rich protein 14) was firstly identified for its ability to specify and localize heterochromatin during cell cycle progression. Aberrant expression of PRR14 is associated with the tumorigenesis and progression of lung cancer. However, its involvement in colon cancer remains unknown. Herein, we report the role of PRR14 in colon cancer.MethodsColon cancer tissue microarray was used to analyze and compare the expression of PRR14 among some clinicopathological characteristics of colon cancer. HCT116 and RKO cells were transfected with siRNA to downregulate PRR14 expression. The roles of PRR14 in proliferation, migration and invasion of the cell lines were determined using cell counting kit-8, colony formation assay, wound healing assay and transwell assays respectively. The expression of PRR14 was measured by using immunofluorescence, qRT- PCR and western blot.ResultsPRR14 was highly expressed in colon cancer tissues, and the expression level was correlated with tumor size, distant metastasis and Tumor Node Metastasis stages. Functional study revealed that downregulation of PRR14 inhibited colon cancer cells growth, migration and invasion. Furthermore, knockdown of PRR14 inhibited epithelial-mesenchymal transition (EMT) process, cell cycle-associated proteins expression and p-AKT level.ConclusionPRR14 may promote the progression and metastasis of colon cancer, and may be a novel prognostic and therapeutic marker for the disease.
- Subjects :
- 0301 basic medicine
Male
Colorectal cancer
Chromosomal Proteins, Non-Histone
Cell
Apoptosis
medicine.disease_cause
Biochemistry
Metastasis
Mice
0302 clinical medicine
Basic Cancer Research
Medicine and Health Sciences
Small interfering RNAs
Cell Cycle and Cell Division
Neoplasm Metastasis
Multidisciplinary
Tissue microarray
Cell Death
Cell Cycle
Cell cycle
Cancer Cell Migration
Gene Expression Regulation, Neoplastic
Nucleic acids
Cell Motility
medicine.anatomical_structure
Oncology
Cell Processes
030220 oncology & carcinogenesis
Colonic Neoplasms
Medicine
Female
Signal Transduction
Research Article
Epithelial-Mesenchymal Transition
Science
Mice, Nude
Cell Migration
Biology
03 medical and health sciences
medicine
Biomarkers, Tumor
Genetics
Animals
Humans
Epithelial–mesenchymal transition
Non-coding RNA
Cell Cycle Inhibitors
PI3K/AKT/mTOR pathway
Colorectal Cancer
Biology and life sciences
Cell growth
Cancers and Neoplasms
Cell Biology
medicine.disease
HCT116 Cells
Gene regulation
030104 developmental biology
Cancer research
RNA
Gene expression
Carcinogenesis
Proto-Oncogene Proteins c-akt
Developmental Biology
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 14
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....f21eba390e1577c2ce74b9ea55dbe7bb