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Residue depletion of oxytetracycline (OTC) and 4-epi-oxytetracycline (4-epi-OTC) in broiler chicken's claws by liquid chromatography-tandem mass spectrometry (LC-MS/MS)
- Source :
- Food additivescontaminants. Part A, Chemistry, analysis, control, exposurerisk assessment. 34(4)
- Publication Year :
- 2016
-
Abstract
- Antibiotics are widely used in poultry production for the treatment of bacterial diseases. However, residues may remain in products and by-products destined for human consumption or animal feeding. The claws of chickens, which are a by-product of the poultry industry, can directly or indirectly enter the food chain as meals destined to feed other productive animals. Thus, it becomes necessary to determine and quantify antimicrobial residues present in this matrix. The objective of the study was to assess the depletion of oxytetracycline (OTC) and its metabolite 4-epi-OTC in broiler chicken's claws. Claws of 32 broilers treated with a therapeutic dosage of 10% OTC during 7 days were analysed. Samples were taken at days 3, 9, 15 and 19 post-treatment. As for the control group, eight broiler chickens were raised under the same conditions. Extraction was carried out through EDTA-McIlvaine buffer, and clean-up employed a SPE C-18 Sep-Pak®. Instrumental analysis was performed through LC-MS/MS. The concentrations of both analytes were determined in claw samples until day 19 post-treatment. Average concentrations were within the LOD (20 μg kg-1) and LOQ (22 µg kg-1) for OTC and 84 μg kg-1 for 4-epi-OTC. Withdrawal times (WDTs) of 39 days for OTC and 54 days for 4-epi-OTC were established in claws based on 95% confidence. These findings demonstrate that claws can be a source of antimicrobial residue entry into the food chain, since the results showed that OTC and its metabolite can be found in chicken's claws for long periods, even exceeding the average lifespan of a broiler chicken.
- Subjects :
- Claw
Hoof and Claw
animal structures
Food Chain
Animal feed
Health, Toxicology and Mutagenesis
Metabolite
Oxytetracycline
Toxicology
01 natural sciences
chemistry.chemical_compound
0404 agricultural biotechnology
Liquid chromatography–mass spectrometry
Limit of Detection
Tandem Mass Spectrometry
medicine
Animals
Humans
Solid phase extraction
Food science
Chile
Biotransformation
business.industry
010401 analytical chemistry
Solid Phase Extraction
Public Health, Environmental and Occupational Health
Broiler
04 agricultural and veterinary sciences
General Chemistry
General Medicine
Poultry farming
040401 food science
Animal Feed
Drug Residues
0104 chemical sciences
Biotechnology
Anti-Bacterial Agents
chemistry
business
Chickens
Food Science
medicine.drug
Chromatography, Liquid
Subjects
Details
- ISSN :
- 19440057
- Volume :
- 34
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Food additivescontaminants. Part A, Chemistry, analysis, control, exposurerisk assessment
- Accession number :
- edsair.doi.dedup.....f205e1c0488318840bb77dd8131c2afd