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Comprehensive transcriptome profiling reveals abundant long nonācoding <scp>RNAs</scp> associated with development of the rice false smut fungus, Ustilaginoidea virens
- Source :
- Environmental Microbiology. 23:4998-5013
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Long non-coding RNAs (lncRNAs) play an important role in biological processes but regulation and function of lncRNAs remain largely unelucidated, especially in fungi. Ustilaginoidea virens is an economically important fungus causing a devastating disease of rice. By combining microscopic and RNA-seq analyses, we comprehensively characterized lncRNAs of this fungus in infection and developmental processes and defined four serial typical stages. RNA-seq analyses revealed 1724 lncRNAs in U. virens, including 1084 long intergenic non-coding RNAs (lincRNAs), 51 intronic RNAs (incRNAs), 566 natural antisense transcripts (lncNATs) and 23 sense transcripts. Gene Ontology enrichment of differentially expressed lincRNAs and lncNATs demonstrated that these were mainly involved in transport-related regulation. Functional studies of transport-related lncRNAs revealed that UvlncNAT-MFS, a cytoplasm localized lncNAT of a putative MFS transporter gene, UvMFS, could form an RNA duplex with UvMFS and was required for regulation of growth, conidiation and various stress responses. Our results were the first to elucidate the lncRNA profiles during infection and development of this important phytopathogen U. virens. The functional discovery of the novel lncRNA, UvlncNAT-MFS, revealed the potential of lncRNAs in regulation of life processes in fungi.
- Subjects :
- Genetics
0303 health sciences
biology
030306 microbiology
Gene Expression Profiling
Ustilaginoidea virens
Conidiation
RNA
Oryza
Fungus
biology.organism_classification
Microbiology
03 medical and health sciences
Intergenic region
Hypocreales
Sense (molecular biology)
Transcriptome profiling
RNA, Long Noncoding
Transcriptome
Ecology, Evolution, Behavior and Systematics
Function (biology)
Biological Phenomena
030304 developmental biology
Subjects
Details
- ISSN :
- 14622920 and 14622912
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Environmental Microbiology
- Accession number :
- edsair.doi.dedup.....f1fcc6c14fd5509091308815c16cf67c