Back to Search
Start Over
Tanshinone IIA attenuates TNF-α induced PTX3 expression and monocyte adhesion to endothelial cells through the p38/NF-κB pathway
- Source :
- Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 121
- Publication Year :
- 2018
-
Abstract
- Tanshinone IIA is one of the most predominant bioactive constituents of Danshen, a traditional Chinese medicinal plant with multiple cardiovascular protective actions. Although Tanshinone IIA has been well documented for its endothelial protective efficacy, studies unveiling the mechanism and/or molecular targets for its pharmacological activity are still inadequate. In recent studies, it has been envisaged that the expression of pentraxin 3 (PTX3) was associated with atherosclerotic cardiovascular diseases (ACVD). Therefore, the current study was designed to evaluate the possible role of Tanshinone IIA in influencing the expression of PTX3 in endothelial cells and thereby prevents endothelial dysfunction. Molecular analyses through real-time PCR, western blot, and ELISA revealed that Tanshinone IIA down-regulates PTX3 gene expression as well as protein secretion in human endothelial cells in the presence or absence of TNF-α. Besides, Tanshinone IIA inhibits the adhesion of THP1 cells (a monocytic cell line) to activated-endothelial cells stimulated with TNF-α. Furthermore, mechanistic studies uncovered the role of p38 MAPK/NF-κB pathway in Tanshinone II-A mediated pharmacological effects. Thus, the present study exemplifies the manifestation of Tanshinone IIA as a plausible alternative natural remedy for ACVD by targeting PTX3.
- Subjects :
- 0301 basic medicine
Endothelium
Cell Survival
MAP Kinase Signaling System
p38 mitogen-activated protein kinases
030204 cardiovascular system & hematology
Toxicology
Monocytes
Cell Line
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Western blot
medicine
Cell Adhesion
Humans
Endothelial dysfunction
medicine.diagnostic_test
Molecular Structure
Tumor Necrosis Factor-alpha
NF-kappa B
Endothelial Cells
NF-κB
Biological activity
General Medicine
PTX3
medicine.disease
Serum Amyloid P-Component
030104 developmental biology
medicine.anatomical_structure
C-Reactive Protein
chemistry
Gene Expression Regulation
Cell culture
Abietanes
Cancer research
RNA Interference
Food Science
Subjects
Details
- ISSN :
- 18736351
- Volume :
- 121
- Database :
- OpenAIRE
- Journal :
- Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
- Accession number :
- edsair.doi.dedup.....f1e0ccf9408fa6dc575c63c4828d191c