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Organization and nucleotide sequence of the Streptococcus mutans galactose operon
- Source :
- Gene. 180:137-144
- Publication Year :
- 1996
- Publisher :
- Elsevier BV, 1996.
-
Abstract
- The galactose operon encoding a repressor and genes for the Leloir pathway for galactose metabolism (galactokinase, galactose-1-phosphate-uridyl transferase and UDP glucose-4-epimerase) was located adjacent to the multiple sugar metabolism (msm) operon on the chromosome of Streptococcus mutans Ingbritt (serotype c) and the complete nucleotide sequence of this 5-kilobase region was determined. The Leloir pathway was induced by the presence of galactose in the growth medium or following the release of intracellular galactose after uptake and cleavage of α-galactosides by the multiple sugar metabolism system. Analysis of the mechanism of galactose transport confirmed the absence of a galactose-specific phosphotransferase system and suggested the presence of an inducible galactose permease. Evidence is presented that galactose transport is independent of the proton motive force and may be ATP-dependent.
- Subjects :
- Operon
Molecular Sequence Data
Biological Transport, Active
Gene Expression
Biology
Streptococcus mutans
chemistry.chemical_compound
Adenosine Triphosphate
Escherichia coli
Genetics
gal operon
Amino Acid Sequence
Cloning, Molecular
Galactose transport
Base Sequence
Sequence Homology, Amino Acid
Permease
Genetic Complementation Test
Galactose
DNA
General Medicine
PEP group translocation
Galactokinase
Leloir pathway
Mutagenesis, Insertional
chemistry
Biochemistry
Energy Metabolism
Subjects
Details
- ISSN :
- 03781119
- Volume :
- 180
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....f1d878c9ade36dde398b49ce520afca8