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Tannic acid as a plant-derived polyphenol exerts vasoprotection via enhancing KLF2 expression in endothelial cells
- Source :
- Scientific Reports, Vol 7, Iss 1, Pp 1-9 (2017), Scientific Reports
- Publication Year :
- 2017
- Publisher :
- Nature Portfolio, 2017.
-
Abstract
- The transcription factor Kruppel-like factor 2 (KLF2) is a critical anti-inflammatory and anti-atherogenic molecule in vascular endothelium. Enhancing KLF2 expression and activity improves endothelial function and prevents atherosclerosis. However, the pharmacological and molecular regulators for KLF2 are scarce. Using high-throughput luciferase reporter assay to screen for KLF2 activators, we have identified tannic acid (TA), a polyphenolic compound, as a potent KLF2 activator that attenuates endothelial inflammation. Mechanistic studies suggested that TA induced KLF2 expression in part through the ERK5/MEF2 pathway. Functionally, TA markedly decreased monocyte adhesion to ECs by reducing expression of adhesion molecule VCAM1. Using lung ECs isolated from Klf2+/+ and Klf2+/− mice, we showed that the anti-inflammatory effect of TA is dependent on KLF2. Collectively, our results demonstrate that TA is a potent KLF2 activator and TA attenuated endothelial inflammation through upregulation of KLF2. Our findings provide a novel mechanism for the well-established beneficial cardiovascular effects of TA and suggest that KLF2 could be a novel therapeutic target for atherosclerotic vascular disease.
- Subjects :
- 0301 basic medicine
Mef2
Endothelium
Science
Anti-Inflammatory Agents
Drug Evaluation, Preclinical
Kruppel-Like Transcription Factors
Inflammation
Biology
Protective Agents
Models, Biological
Monocytes
Article
03 medical and health sciences
chemistry.chemical_compound
Mice
Downregulation and upregulation
Tannic acid
medicine
Cell Adhesion
Human Umbilical Vein Endothelial Cells
Animals
Humans
Transcription factor
Multidisciplinary
Activator (genetics)
Tumor Necrosis Factor-alpha
Polyphenols
Cell biology
030104 developmental biology
medicine.anatomical_structure
Biochemistry
chemistry
KLF2
Medicine
Endothelium, Vascular
medicine.symptom
Tannins
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 7
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....f1cd895f06e77a17faf453c33f469c58