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Emergence of Bacterial Soft Rot in Cucumber Caused by Pectobacterium carotovorum subsp. brasiliense in China
- Source :
- Plant disease. 101(2)
- Publication Year :
- 2019
-
Abstract
- During 2014 to 2015, a devastating bacterial soft rot on cucumber stems and leaves occurred in Shandong, Shanxi, Hebei, Henan, and Liaoning provinces of China, resulting in serious economic losses for cucumber production. The gummosis emerged on the surface of leaves, stems, petioles, and fruit of cucumber. The basal stem color was dark brown and the stem base turned to wet rot. Yellow spots and wet rot emerged at the edge of the infected cucumber leaves and gradually infected the leaf centers. In total, 45 bacterial strains were isolated from the infected tissues. On the basis of phenotypic properties of morphology, physiology, biochemistry, and 16S ribosomal RNA gene sequence analysis, the pathogen was identified as Pectobacterium carotovorum. Multilocus sequence analysis confirmed that the isolates were P. carotovorum subsp. brasiliense, and the pathogens fell in clade II. The pathogenicity of isolated bacteria strains was confirmed. The strains reisolated were the same as the original. The host range test confirmed that strains had a wide range of hosts. As far as we know, this is the first report of cucumber stem soft rot caused by P. carotovorum subsp. brasiliense in China as well as in the world, which has a significant economic impact on cucumber production.
- Subjects :
- 0106 biological sciences
0301 basic medicine
biology
Spots
Sequence analysis
Gummosis
Pectobacterium carotovorum
Plant Science
biology.organism_classification
16S ribosomal RNA
01 natural sciences
03 medical and health sciences
Bacterial soft rot
030104 developmental biology
Botany
Agronomy and Crop Science
Pathogen
Bacteria
010606 plant biology & botany
Subjects
Details
- ISSN :
- 01912917
- Volume :
- 101
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Plant disease
- Accession number :
- edsair.doi.dedup.....f1b906c5b71b1d4f2999d2380aec631d