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Human .alpha.-fetoprotein primary structure: a mass spectrometric study
- Source :
- Biochemistry. 30:5061-5066
- Publication Year :
- 1991
- Publisher :
- American Chemical Society (ACS), 1991.
-
Abstract
- The amino acid sequence of human alpha-fetoprotein, a 67-kDa protein present in mammalian embryonic serum, was verified by fast atom bombardment mass spectrometric (FAB/MS) analyses of three different enzymatic digests of the protein. Human alpha-fetoprotein obtained from a large-scale cell culture was digested with trypsin and V-8 protease either separately on two different samples or combined on the same one. The V-8 protease digest of the protein was partially fractionated by HPLC; the other samples were directly analyzed by FAB/MS without previous purification steps. About 90% of the alpha-fetoprotein amino acid sequence was verified by mass spectrometric analysis; this also confirmed that the cell-derived protein is identical with the hepatoma-derived protein. FAB analysis revealed that the N terminus of the mature protein is arginine rather than threonine, with the threonine occupying the second position. Therefore, the processing site of the alpha-fetoprotein signal peptide during maturation of the protein occurs at the N-terminal side of the arginine residue formerly indicated as residue-1. Thus mature alpha-fetoprotein contains 591 amino acids rather than 590.
- Subjects :
- medicine.medical_treatment
Molecular Sequence Data
Spectrometry, Mass, Fast Atom Bombardment
Biochemistry
Cell Line
Mice
Residue (chemistry)
Sequence Homology, Nucleic Acid
medicine
Animals
Humans
Amino Acid Sequence
Threonine
Peptide sequence
Chromatography, High Pressure Liquid
chemistry.chemical_classification
Protease
Chromatography
Chemistry
Protein primary structure
Fast atom bombardment
Trypsin
Peptide Fragments
Rats
Amino acid
alpha-Fetoproteins
medicine.drug
Subjects
Details
- ISSN :
- 15204995 and 00062960
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Biochemistry
- Accession number :
- edsair.doi.dedup.....f1b79d3e8e5bf7581697922af8152523