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A Single Legionella Effector Catalyzes a Multistep Ubiquitination Pathway to Rearrange Tubular Endoplasmic Reticulum for Replication
- Source :
- Cell Host & Microbe. 21:169-181
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Intracellular pathogens manipulate host organelles to support replication within cells. For Legionella pneumophila, the bacterium translocates proteins that establish an endoplasmic reticulum (ER)-associated replication compartment. We show here that the bacterial Sde proteins target host reticulon 4 (Rtn4) to control tubular ER dynamics, resulting in tubule rearrangements as well as alterations in Rtn4 associated with the replication compartment. These rearrangements are triggered via Sde-promoted ubiquitin transfer to Rtn4, occurring almost immediately after bacterial uptake. Ubiquitin transfer requires two sequential enzymatic activities from a single Sde polypeptide: an ADP-ribosyltransferase and a nucleotidase/phosphohydrolase. The ADP-ribosylated moiety of ubiquitin is a substrate for the nucleotidase/phosphohydrolase, resulting in either transfer of ubiquitin to Rtn4, or phosphoribosylation of ubiquitin in the absence of a ubiquitination target. Therefore, a single bacterial protein drives a multistep biochemical pathway to control ubiquitination and tubular ER function independently of the host ubiquitin machinery.
- Subjects :
- 0301 basic medicine
Nogo Proteins
Biology
Ubiquitin-conjugating enzyme
Endoplasmic Reticulum
Microbiology
Legionella pneumophila
Article
Catalysis
03 medical and health sciences
Bacterial Proteins
Ubiquitin
Virology
Chlorocebus aethiops
Serine
Animals
Humans
ADP Ribose Transferases
Gene Rearrangement
Adenosine Diphosphate Ribose
Bacteria
Effector
Endoplasmic reticulum
Ubiquitination
biology.organism_classification
Cell biology
Ubiquitin ligase
HEK293 Cells
030104 developmental biology
Biochemistry
Reticulon
COS Cells
Host-Pathogen Interactions
biology.protein
Parasitology
HeLa Cells
Reticulon 4
Subjects
Details
- ISSN :
- 19313128
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Cell Host & Microbe
- Accession number :
- edsair.doi.dedup.....f194e431ca3032cdbfbae210b7dcb03f