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Nitro-imidazole-based ruthenium complexes with antioxidant and anti-inflammatory activities
- Source :
- Journal of inorganic biochemistry. 206
- Publication Year :
- 2019
-
Abstract
- Inflammation is a physiological process triggered in response to tissue damage, and involves events related to cell recruitment, cytokines release and reactive oxygen species (ROS) production. Failing to control the process duration lead to chronification and may be associated with the development of various pathologies, including autoimmune diseases and cancer. Considering the pharmacological potential of metal-based compounds, two new ruthenium complexes were synthesized: cis-[Ru(NO2)(bpy)2(5NIM)]PF6 (1) and cis-[RuCl(bpy)2(MTZ)]PF6 (2), where bpy = 2,2′-bipyridine, 5NIM = 5-nitroimidazole and MTZ = metronidazole. Both products were characterized by spectroscopic techniques, followed by Density Functional Theory (DFT) calculations in order to support experimental findings. Afterwards, their in vitro cytotoxic, antioxidant and anti-inflammatory activities were investigated. Compounds 1 and 2 presented expressive in vitro antioxidant activity, reducing lipid peroxidation and decreasing intracellular ROS levels with comparable effectiveness to the standard steroidal drug dexamethasone or α-tocopherol. These complexes showed no noticeable cytotoxicity on the tested cancer cell lines. Bactericidal assay against metronidazole-resistant Helicobacter pylori, a microorganism able to disrupt oxidative balance, unraveled compound 1 moderate activity over that strain. Besides this, it was able to inhibit interleukin-6 (IL-6) and tumor necrosis factor-α (TNF- α) production as well as interleukin-1β (IL-1β) and cyclooxygenase-2 (COX-2) expression in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. This latter activity is remarkable, which has not been reported for other ruthenium-based complexes. Altogether, these results suggest cis-[Ru(NO2)(bpy)2(5NIM)]PF6 complex has potential pharmacological application as an anti-inflammatory agent that deserve further biological investigation.
- Subjects :
- Antioxidant
Lipopolysaccharide
medicine.drug_class
medicine.medical_treatment
Anti-Inflammatory Agents
chemistry.chemical_element
Antineoplastic Agents
Pharmacology
010402 general chemistry
01 natural sciences
Biochemistry
Anti-inflammatory
Antioxidants
Ruthenium
Inorganic Chemistry
Lipid peroxidation
chemistry.chemical_compound
Mice
Coordination Complexes
Superoxides
medicine
Animals
Humans
Cytotoxicity
Cell Proliferation
chemistry.chemical_classification
Reactive oxygen species
Bacteria
Molecular Structure
010405 organic chemistry
Imidazoles
0104 chemical sciences
Anti-Bacterial Agents
RAW 264.7 Cells
chemistry
A549 Cells
MCF-7 Cells
Tumor necrosis factor alpha
Lipid Peroxidation
Subjects
Details
- ISSN :
- 18733344
- Volume :
- 206
- Database :
- OpenAIRE
- Journal :
- Journal of inorganic biochemistry
- Accession number :
- edsair.doi.dedup.....f19029b8ff70d5c3664047037739ea31