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Single-cell RNA sequencing of developing maize ears facilitates functional analysis and trait candidate gene discovery
- Source :
- Dev Cell
- Publication Year :
- 2020
-
Abstract
- Crop productivity depends on activity of meristems that produce optimized plant architectures, including that of the maize ear. A comprehensive understanding of development requires insight into the full diversity of cell types and developmental domains and the gene networks required to specify them. Until now, these were identified primarily by morphology and insights from classical genetics, which are limited by genetic redundancy and pleiotropy. Here, we investigated the transcriptional profiles of 12,525 single cells from developing maize ears. The resulting developmental atlas provides a single-cell RNA sequencing (scRNA-seq) map of an inflorescence. We validated our results by mRNA in situ hybridization and by fluorescence-activated cell sorting (FACS) RNA-seq, and we show how these data may facilitate genetic studies by predicting genetic redundancy, integrating transcriptional networks, and identifying candidate genes associated with crop yield traits.
- Subjects :
- Candidate gene
Quantitative Trait Loci
Gene regulatory network
Genome-wide association study
Computational biology
Biology
Zea mays
General Biochemistry, Genetics and Molecular Biology
Article
03 medical and health sciences
0302 clinical medicine
Pleiotropy
Gene Expression Regulation, Plant
Gene Regulatory Networks
Molecular Biology
Genetic Association Studies
030304 developmental biology
0303 health sciences
Base Sequence
Sequence Analysis, RNA
Protoplasts
RNA
Gene Expression Regulation, Developmental
Reproducibility of Results
Cell Biology
Cell sorting
Single cell sequencing
Genetic redundancy
Single-Cell Analysis
Transcriptome
030217 neurology & neurosurgery
Developmental Biology
Subjects
Details
- ISSN :
- 18781551
- Volume :
- 56
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Developmental cell
- Accession number :
- edsair.doi.dedup.....f184a21b8886112c26072f826cd2440c