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Schistosoma mansoni: resistant specific infection-induced gene expression in Biomphalaria glabrata identified by fluorescent-based differential display
- Source :
- Experimental Parasitology. 107:97-104
- Publication Year :
- 2004
- Publisher :
- Elsevier BV, 2004.
-
Abstract
- The freshwater tropical snail Biomphalaria glabrata is an intermediate host for Schistosoma mansoni, the causative agent of human intestinal schistosomiasis, and strains differ in their susceptibility to parasite infection. Changes in gene expression in response to parasite infection have been simultaneously examined in a susceptible strain (NHM1742) and a resistant strain (NHM1981) using a newly developed fluorescent-based differential display method. Such RNA profiling techniques allow the examination of changes in gene expression in response to parasite infection, without requiring previous sequence knowledge, or selecting candidate genes that may be involved in the complex neuroendocrine or defence systems of the snail. Thus, novel genes may be identified. Ten transcripts were initially identified, present only in the profiles derived from snails of the resistant strain when exposed to infection. The differential expression of five of these genes, including HSP70 and several novel transcripts with one containing at least two globin-like domains, has been confirmed by semi-quantitative RT-PCR.
- Subjects :
- DNA, Complementary
Molecular Sequence Data
Immunology
Gene Expression
Biomphalaria
Snail
Biology
Host-Parasite Interactions
Microbiology
biology.animal
parasitic diseases
Gene expression
Animals
Biomphalaria glabrata
Amino Acid Sequence
Gene
DNA Primers
Differential display
Myoglobin
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Schistosoma mansoni
General Medicine
Blotting, Northern
biology.organism_classification
Molecular biology
Up-Regulation
Gene expression profiling
Infectious Diseases
RNA
Parasitology
Subjects
Details
- ISSN :
- 00144894
- Volume :
- 107
- Database :
- OpenAIRE
- Journal :
- Experimental Parasitology
- Accession number :
- edsair.doi.dedup.....f148acf99ce4be0ec1f181802dfd07b8