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Mechanistic insights into the digestion of complex dietary fibre by the rumen microbiota using combinatorial high-resolution glycomics and transcriptomic analyses
- Source :
- Computational and Structural Biotechnology Journal, Computational and Structural Biotechnology Journal, Vol 20, Iss, Pp 148-164 (2022)
- Publication Year :
- 2022
- Publisher :
- Elsevier BV, 2022.
-
Abstract
- Graphical abstract<br />There is a knowledge gap regarding the factors that impede the ruminal digestion of plant cell walls or if rumen microbiota possess the functional activities to overcome these constraints. Innovative experimental methods were adopted to provide a high-resolution understanding of plant cell wall chemistries, identify higher-order structures that resist microbial digestion, and determine how they interact with the functional activities of the rumen microbiota. We characterized the total tract indigestible residue (TTIR) from cattle fed a low-quality straw diet using two comparative glycomic approaches: ELISA-based glycome profiling and total cell wall glycosidic linkage analysis. We successfully detected numerous and diverse cell wall glycan epitopes in barley straw (BS) and TTIR and determined their relative abundance pre- and post-total tract digestion. Of these, xyloglucans and heteroxylans were of higher abundance in TTIR. To determine if the rumen microbiota can further saccharify the residual plant polysaccharides within TTIR, rumen microbiota from cattle fed a diet containing BS were incubated with BS and TTIR ex vivo in batch cultures. Transcripts coding for carbohydrate-active enzymes (CAZymes) were identified and characterized for their contribution to cell wall digestion based on glycomic analyses, comparative gene expression profiles, and associated CAZyme families. High-resolution phylogenetic fingerprinting of these sequences encoded CAZymes with activities predicted to cleave the primary linkages within heteroxylan and arabinan. This experimental platform provides unprecedented precision in the understanding of forage structure and digestibility, which can be extended to other feed-host systems and inform next-generation solutions to improve the performance of ruminants fed low-quality forages.
- Subjects :
- AG, arabinogalactan
Nutrient utilization
GH, glycosyl hydrolase
Rumen microbiome
AB, arabinan
Biophysics
Glycome profiling
Biochemistry
AO, ammonium oxalate
CAZyme, carbohydrate active enzyme
PC, post-chlorite
Structural Biology
PL, polysaccharide lyase
Genetics
XG, xyloglucan
NDF, neutral detergent fibre
HX, heteroxylan
Differential gene expression
BS, barley straw
Dietary polysaccharides
ComputingMethodologies_COMPUTERGRAPHICS
AGP, arabinogalactan protein
CE, carbohydrate esterase
TTIR, total tract indigestible residue
food and beverages
ADF, acid detergent fibre
HG, homogalacturonan
ELISA, enzyme-linked immunosorbent assay
AX, arabinoxylan
DE, differentially expressed
AIR, alcohol insoluble residue
HPAEC-PAD, high performance anion exchange chromatography coupled with pulsed amperometric detection
Computer Science Applications
RG, rhamnogalacturonan
FID, flame ionization detection GC, gas chromatography
MS, mass spectrometry
Glycoside hydrolase
SC, sodium carbonate
CH, chlorite
Transcriptome
TP248.13-248.65
CAZymes
Linkage analysis
mAbs, monoclonal antibodies
Research Article
Biotechnology
Subjects
Details
- ISSN :
- 20010370
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Computational and Structural Biotechnology Journal
- Accession number :
- edsair.doi.dedup.....f13e6b8708336992febbf16f29ff0d21