Back to Search
Start Over
Identification and culture of proliferative cells in abnormal Taenia solium larvae: Role in the development of racemose neurocysticercosis
- Source :
- PLoS Neglected Tropical Diseases, PLoS Neglected Tropical Diseases, Vol 15, Iss 3, p e0009303 (2021), CONCYTEC-Institucional, Consejo Nacional de Ciencia Tecnología e Innovación Tecnológica, instacron:CONCYTEC
- Publication Year :
- 2020
-
Abstract
- Racemose neurocysticercosis is an aggressive disease caused by the aberrant expansion of the cyst form of Taenia solium within the subarachnoid spaces of the human brain and spinal cord resulting in a mass effect and chronic inflammation. Although expansion is likely caused by the proliferation and growth of the parasite bladder wall, there is little direct evidence of the mechanisms that underlie these processes. Since the development and growth of cysts in related cestodes involves totipotential germinative cells, we hypothesized that the expansive growth of the racemose larvae is organized and maintained by germinative cells. Here, we identified proliferative cells expressing the serine/threonine-protein kinase plk1 by in situ hybridization. Proliferative cells were present within the bladder wall of racemose form and absent from the homologous tissue surrounding the vesicular form. Cyst proliferation in the related model species Taenia crassiceps (ORF strain) occurs normally by budding from the cyst bladder wall and proliferative cells were concentrated within the growth buds. Cells isolated from bladder wall of racemose larvae were established in primary cell culture and insulin stimulated their proliferation in a dose-dependent manner. These findings indicate that the growth of racemose larvae is likely due to abnormal cell proliferation. The different distribution of proliferative cells in the racemose larvae and their sensitivity to insulin may reflect significant changes at the cellular and molecular levels involved in their tumor-like growth. Parasite cell cultures offer a powerful tool to characterize the nature and formation of the racemose form, understand the developmental biology of T. solium, and to identify new effective drugs for treatment.<br />Author summary Racemose neurocysticercosis is the most aggressive and lethal form of the disease characterized by progressive expansion of the larval stage of Taenia solium within the subarachnoid spaces of the brain. Currently, the cellular or molecular events that promote its formation and development are unknown. Our observations, employing tissue samples, revealed the presence of mitotically active cells in the bladder wall of the racemose larvae. Finally, we isolated and established primary cell cultures and observed that these cells are sensitive (proliferate in response to insulin). These results suggest that the proliferative cells stimulated by host biomolecules like hormones would be responsible for the abnormal growth of racemose larvae. Further studies are needed to identify all genes and pathways altered in the racemose form. The primary cell cultures will be used as a therapeutic target and allow us in future works to understand the developmental biology of the parasite.
- Subjects :
- 0301 basic medicine
Life Cycles
RC955-962
Neurocysticercosis
Flatworms
Cell Culture Techniques
complementary DNA
Gene Expression
Parasitic intestinal diseases
Biochemistry
0302 clinical medicine
Larvae
Endocrinology
Medical Conditions
Arctic medicine. Tropical medicine
Taenia solium
Medicine and Health Sciences
Insulin
Cyst
Cancers and neoplasms
biology
Brain
Eukaryota
beta actin
Cell biology
medicine.drug_formulation_ingredient
Infectious Diseases
Larva
Biological Cultures
Public aspects of medicine
RA1-1270
medicine.symptom
Anatomy
parasite antigen
purl.org/pe-repo/ocde/ford#3.03.06 [https]
Research Article
Cell Culturing Techniques
Bladder
030231 tropical medicine
Primary Cell Culture
Inflammation
In situ hybridization
Research and Analysis Methods
03 medical and health sciences
protein serine threonine kinase
Helminths
parasitic diseases
medicine
Parasitic Diseases
Genetics
Animals
Humans
Primary cell culture
Cell Proliferation
Taenia crassiceps
Diabetic Endocrinology
polo like kinase 1
Cestodes
Public Health, Environmental and Occupational Health
Organisms
Biology and Life Sciences
Renal System
Cell Cultures
Cell cultures
biology.organism_classification
medicine.disease
Invertebrates
Hormones
030104 developmental biology
Cell culture
Antigens, Helminth
Parasitic Intestinal Diseases
Developmental biology
Zoology
purl.org/pe-repo/ocde/ford#4.04.01 [http]
Developmental Biology
Subjects
Details
- ISSN :
- 19352735
- Volume :
- 15
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PLoS neglected tropical diseases
- Accession number :
- edsair.doi.dedup.....f12dbbf7412bed6af0d0941a5b8a782a