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Lower respiratory tract myeloid cells harbor SARS-CoV-2 and display an inflammatory phenotype
- Source :
- Chest, medRxiv
- Publication Year :
- 2020
- Publisher :
- Cold Spring Harbor Laboratory, 2020.
-
Abstract
- SARS-CoV-2 pneumonia may induce an aberrant immune response with brisk recruitment of myeloid cells into the lower respiratory tract, which may contribute to morbidity and mortality. We describe endotracheal aspirate samples from seven patients with SARS-CoV-2 pneumonia requiring mechanical ventilation. We note SARS-CoV-2 virions within lower respiratory tract myeloid cells shown by electron tomography, immunofluorescence confocal imaging, and immuno-electron microscopy. Endotracheal aspirates are primarily composed of mononuclear and polymorphonuclear leukocytes. These myeloid cells that harbor virus are frequently positive for CD14 and/or CD16 and most display an inflammatory phenotype marked by expression of IL-6 and tissue factor mRNA transcript and protein expression.
- Subjects :
- Male
Electron Microscope Tomography
Neutrophils
viruses
Antibodies, Viral
Virus Replication
Critical Care and Intensive Care Medicine
RT-PCR, reverse transcriptase polymerase chain reaction
0302 clinical medicine
Medicine
Myeloid Cells
030212 general & internal medicine
skin and connective tissue diseases
Lung
medicine.diagnostic_test
virus diseases
Middle Aged
Phenotype
medicine.anatomical_structure
Female
Cardiology and Cardiovascular Medicine
IRB, institutional review board
Pulmonary and Respiratory Medicine
CD14
Pneumonia, Viral
CD, cluster of differentiation
SARS-CoV, severe acute respiratory syndrome coronavirus
Respiratory Mucosa
Immunofluorescence
Article
Virus
03 medical and health sciences
Tissue factor
Immune system
Research Letter
Coronavirus Nucleocapsid Proteins
Humans
Immunity, Mucosal
BD, Becton Dickinson
SARS-CoV-2
business.industry
ETA, endotracheal aspirate
fungi
COVID-19
Phosphoproteins
medicine.disease
Respiration, Artificial
respiratory tract diseases
IL, interleukin
Microscopy, Electron
Pneumonia
030228 respiratory system
Immunology
business
Respiratory tract
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Chest, medRxiv
- Accession number :
- edsair.doi.dedup.....f11b90f8eaf02a0fff6aac0f3afc3789
- Full Text :
- https://doi.org/10.1101/2020.08.11.20171967