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Whole Genome Mapping and Re-Organization of the Nuclear and Mitochondrial Genomes of Babesia microti Isolates
- Source :
- PLoS ONE, PLoS ONE, Public Library of Science, 2013, 8 (9), pp.e72657. ⟨10.1371/journal.pone.0072657⟩, PloS One, PLoS ONE, Vol 8, Iss 9, p e72657 (2013)
- Publication Year :
- 2013
- Publisher :
- HAL CCSD, 2013.
-
Abstract
- Babesia microti is the primary causative agent of human babesiosis, an emerging pathogen that causes a malaria-like illness with possible fatal outcome in immunocompromised patients. The genome sequence of the B. microti R1 strain was reported in 2012 and revealed a distinct evolutionary path for this pathogen relative to that of other apicomplexa. Lacking from the first genome assembly and initial molecular analyses was information about the terminal ends of each chromosome, and both the exact number of chromosomes in the nuclear genome and the organization of the mitochondrial genome remained ambiguous. We have now performed various molecular analyses to characterize the nuclear and mitochondrial genomes of the B. microti R1 and Gray strains and generated high-resolution Whole Genome maps. These analyses show that the genome of B. microti consists of four nuclear chromosomes and a linear mitochondrial genome present in four different structural types. Furthermore, Whole Genome mapping allowed resolution of the chromosomal ends, identification of areas of misassembly in the R1 genome, and genomic differences between the R1 and Gray strains, which occur primarily in the telomeric regions. These studies set the stage for a better understanding of the evolution and diversity of this important human pathogen.
- Subjects :
- Genome evolution
Mitochondrial DNA
Nuclear gene
[SDV]Life Sciences [q-bio]
lcsh:Medicine
Genomics
Biology
L73 - Maladies des animaux
Babesia microti
Genome
03 medical and health sciences
parasitic diseases
Animals
lcsh:Science
Genome size
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Whole genome sequencing
Genetics
0303 health sciences
Multidisciplinary
030306 microbiology
lcsh:R
Genome project
3. Good health
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Genome, Mitochondrial
lcsh:Q
Genome, Protozoan
L72 - Organismes nuisibles des animaux
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, PLoS ONE, Public Library of Science, 2013, 8 (9), pp.e72657. ⟨10.1371/journal.pone.0072657⟩, PloS One, PLoS ONE, Vol 8, Iss 9, p e72657 (2013)
- Accession number :
- edsair.doi.dedup.....f1064be951e3f7f12eaa62f605c4314d